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First published online January 8, 2004; 10.1104/pp.103.030189

Plant Physiology 134:510-519 (2004)
© 2004 American Society of Plant Biologists

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GENETICS, GENOMICS, AND MOLECULAR EVOLUTION

Increased and Altered Fragrance of Tobacco Plants after Metabolic Engineering Using Three Monoterpene Synthases from Lemon

Joost Lücker1, Wilfried Schwab, Bianca van Hautum, Jan Blaas, Linus H. W. van der Plas, Harro J. Bouwmeester and Harrie A. Verhoeven*

Business Unit Bioscience, Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands (J.L., B.v.H., J.B., H.J.B., H.A.V.); University of Würzburg, Chair of Food Chemistry, Am Hubland, 97974, Würzburg, Germany (W.S.); and Laboratory of Plant Physiology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands (L.H.W.v.d.P.)

Wild-type tobacco (Nicotiana tabacum) plants emit low levels of terpenoids, particularly from the flowers. By genetic modification of tobacco cv Petit Havana SR1 using three different monoterpene synthases from lemon (Citrus limon L. Burm. f.) and the subsequent combination of these three into one plant by crossings, we show that it is possible to increase the amount and alter the composition of the blend of monoterpenoids produced in tobacco plants. The transgenic tobacco plant line with the three introduced monoterpene synthases is emitting {beta}-pinene, limonene, and {gamma}-terpinene and a number of side products of the introduced monoterpene synthases, from its leaves and flowers, in addition to the terpenoids emitted by wild-type plants. The results show that there is a sufficiently high level of substrate accessible for the introduced enzymes.

1 Present address: Biotechnology Laboratory, University of British Columbia, 6174 University Boulevard, Vancouver, British Columbia, Canada V6T 1Z3.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.030189.

* Corresponding author; e-mail harrie.verhoeven{at}wur.nl; fax 31-317418094.

Received July 28, 2003; returned for revision August 10, 2003; accepted September 28, 2003.




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