First published online March 26, 2004; 10.1104/pp.103.036897
Plant Physiology 134:1624-1631 (2004)
© 2004 American Society of Plant Biologists
DEVELOPMENT AND HORMONE ACTION
Brassinosteroids Interact with Auxin to Promote Lateral Root Development in Arabidopsis1
Fang Bao2,
Junjiang Shen2,
Shari R. Brady,
Gloria K. Muday,
Tadao Asami and
Zhenbiao Yang*
Department of Botany and Plant Sciences and Center for Plant Cell Biology, University of California, Riverside, California 92521 (F.B., J.S., Z.Y.); Department of Biology, Wake Forest University, Winston-Salem, NC 27109 (S.R.B., G.K.M.); and Plant Functions Laboratory, RIKEN, Wako, Saitama 351–0198, Japan (T.A.)
Plant hormone brassinosteroids (BRs) and auxin exert some similar physiological effects likely through their functional interaction, but the mechanism for this interaction is unknown. In this study, we show that BRs are required for lateral root development in Arabidopsis and that BRs act synergistically with auxin to promte lateral root formation. BR perception is required for the transgenic expression of the  -glucuronidase gene fused to a synthetic auxin-inducible promoter (DR5::GUS) in root tips, while exogenous BR promotes DR5::GUS expression in the root tips and the stele region proximal to the root tip. BR induction of both lateral root formation and DR5::GUS expression is suppressed by the auxin transport inhibitor N-(1-naphthyl) phthalamic acid. Importantly, BRs promote acropetal auxin transport (from the base to the tip) in the root. Our observations indicate that BRs regulate auxin transport, providing a novel mechanism for hormonal interactions in plants and supporting the hypothesis that BRs promote lateral root development by increasing acropetal auxin transport.
1 This work is supported by the Department of Energy (grant no. DE–FG03–00ER15060/M003 to Z.Y.) and by NASA (grant no. NAG2–1507 to G.M.).
2 These authors contributed equally to the paper.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.036897.
* Corresponding author; e-mail zhenbiao.yang{at}ucr.edu; fax 909–787–4437.
Received December 4, 2003;
returned for revision February 9, 2004;
accepted February 9, 2004.
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