First published online September 3, 2004; 10.1104/pp.104.042903
Plant Physiology 136:2862-2874 (2004)
© 2004 American Society of Plant Biologists
PLANTS INTERACTING WITH OTHER ORGANISMS
The Pepper Transcription Factor CaPF1 Confers Pathogen and Freezing Tolerance in Arabidopsis1
So Young Yi,
Jee-Hyub Kim,
Young-Hee Joung,
Sanghyeob Lee,
Woo-Taek Kim,
Seung Hun Yu and
Doil Choi*
Plant Genomics Laboratory (S.Y.Y., Y.-H.J., S.L., D.C.) and National Center for Genome Information (J.-H.K.), Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejeon 305–600, Korea; Department of Agricultural Biology, Chungnam National University, Taejeon 305–764, Korea (S.Y.Y., S.H.Y.); and Department of Biology, Yonsei University, Seoul 120–749, Korea (W.-T.K.)
An ERF/AP2-type transcription factor (CaPF1) was isolated by differential-display reverse transcription-PCR, following inoculation of the soybean pustule pathogen Xanthomonas axonopodis pv glycines 8ra, which induces hypersensitive response in pepper (Capsicum annuum) leaves. CaPF1 mRNA was induced under conditions of biotic and abiotic stress. Higher levels of CaPF1 transcripts were observed in disease-resistant tissue compared with susceptible tissue. CaPF1 expression was additionally induced using various treatment regimes, including ethephon, methyl jasmonate, and cold stress. To determine the role of CaPF1 in plants, transgenic Arabidopsis and tobacco (Nicotiana tabacum) plants expressing higher levels of CaPF1 were generated. Gene expression analyses of transgenic Arabidopsis and tobacco revealed that the CaPF1 level in transgenic plants affects expression of genes that contain either a GCC or a CRT/DRE box in their promoter regions. Furthermore, transgenic Arabidopsis plants expressing CaPF1 displayed tolerance against freezing temperatures and enhanced resistance to Pseudomonas syringae pv tomato DC3000. Disease tolerance was additionally observed in CaPF1 transgenic tobacco plants. The results collectively indicate that CaPF1 is an ERF/AP2 transcription factor in hot pepper plants that may play dual roles in response to biotic and abiotic stress in plants.
1 This work was supported by grants from the Plant Diversity Research Center, Crop Functional Genomics Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology and the Plant Molecular Genetics and Breeding Research Center through the Korea Science and Engineering Foundation.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.042903.
* Corresponding author; e-mail doil{at}kribb.re.kr; fax 82–42–860–4309.
Received March 17, 2004;
returned for revision May 31, 2004;
accepted May 31, 2004.
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