First published online May 27, 2005; 10.1104/pp.105.060764
Plant Physiology 138:898-908 (2005)
© 2005 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
Characterization of Markers to Determine the Extent and Variability of Leaf Senescence in Arabidopsis. A Metabolic Profiling Approach1
Céline Diaz,
Sarah Purdy,
Aurélie Christ,
Jean-Francois Morot-Gaudry,
Astrid Wingler and
Céline Masclaux-Daubresse*
Unité de Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, 78 026 Versailles cedex, France (C.D., A.C., J.-F.M.-G., C.M.-D.); and Department of Biology, University College London, London WC1E 6BT, United Kingdom (S.P., A.W.)
Comparison of the extent of leaf senescence depending on the genetic background of different recombinant inbred lines (RILs) of Arabidopsis (Arabidopsis thaliana) is described. Five RILs of the Bay-0 x Shahdara population showing differential leaf senescence phenotypes (from early senescing to late senescing) were selected to determine metabolic markers to discriminate Arabidopsis lines on the basis of senescence-dependent changes in metabolism. The proportion of  -aminobutyric acid, leucine, isoleucine, aspartate, and glutamate correlated with (1) the age and (2) the senescence phenotype of the RILs. Differences were observed in the glycine/serine ratio even before any senescence symptoms could be detected in the rosettes. This could be used as predictive indicator for plant senescence behavior. Surprisingly, late-senescing lines appeared to mobilize glutamine, asparagine, and sulfate more efficiently than early-senescing lines. The physiological basis of the relationship between leaf senescence and flowering time was analyzed.
1 This work was supported by the Centre Technique Interprofessionel des Oléagineux Métropolitains (Ph.D. studentship supporting the work of C.D.), the Biotechnology and Biological Siences Research Council (grant no. 31/P16341 to the A.W. laboratory), and the Natural Environment Research Council (Ph.D. studentship supporting the work of S.P.).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.060764.
* Corresponding author; e-mail masclaux{at}versailles.inra.fr; fax 33–(0)1–30–83–30–96.
Received February 3, 2005;
returned for revision April 7, 2005;
accepted April 12, 2005.
This article has been cited by other articles:
|
|
|
|
 
D. Albinsky, M. Kusano, M. Higuchi, N. Hayashi, M. Kobayashi, A. Fukushima, M. Mori, T. Ichikawa, K. Matsui, H. Kuroda, et al.
Metabolomic Screening Applied to Rice FOX Arabidopsis Lines Leads to the Identification of a Gene-Changing Nitrogen Metabolism
Mol Plant,
August 26, 2009;
(2009)
ssp069v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Haudecoeur, S. Planamente, A. Cirou, M. Tannieres, B. J. Shelp, S. Morera, and D. Faure
Proline antagonizes GABA-induced quenching of quorum-sensing in Agrobacterium tumefaciens
PNAS,
August 25, 2009;
106(34):
14587 - 14592.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Abreu and S. Munne-Bosch
Salicylic acid deficiency in NahG transgenic lines and sid2 mutants increases seed yield in the annual plant Arabidopsis thaliana
J. Exp. Bot.,
March 1, 2009;
60(4):
1261 - 1271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H.M. Schippers, A. Nunes-Nesi, R. Apetrei, J. Hille, A. R. Fernie, and P. P. Dijkwel
The Arabidopsis onset of leaf death5 Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing
PLANT CELL,
October 1, 2008;
20(10):
2909 - 2925.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Diaz, T. Lemaitre, A. Christ, M. Azzopardi, Y. Kato, F. Sato, J.-F. Morot-Gaudry, F. Le Dily, and C. Masclaux-Daubresse
Nitrogen Recycling and Remobilization Are Differentially Controlled by Leaf Senescence and Development Stage in Arabidopsis under Low Nitrogen Nutrition
Plant Physiology,
July 1, 2008;
147(3):
1437 - 1449.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Richard-Molard, A. Krapp, F. Brun, B. Ney, F. Daniel-Vedele, and S. Chaillou
Plant response to nitrate starvation is determined by N storage capacity matched by nitrate uptake capacity in two Arabidopsis genotypes
J. Exp. Bot.,
March 1, 2008;
59(4):
779 - 791.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. G. Forde and P. J. Lea
Glutamate in plants: metabolism, regulation, and signalling
J. Exp. Bot.,
July 1, 2007;
58(9):
2339 - 2358.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Masclaux-Daubresse, S. Purdy, T. Lemaitre, N. Pourtau, L. Taconnat, J.-P. Renou, and A. Wingler
Genetic Variation Suggests Interaction between Cold Acclimation and Metabolic Regulation of Leaf Senescence
Plant Physiology,
January 1, 2007;
143(1):
434 - 446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Chevrot, R. Rosen, E. Haudecoeur, A. Cirou, B. J. Shelp, E. Ron, and D. Faure
GABA controls the level of quorum-sensing signal in Agrobacterium tumefaciens
PNAS,
May 9, 2006;
103(19):
7460 - 7464.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Meyer, S. Eskandari, S. Grallath, and D. Rentsch
AtGAT1, a High Affinity Transporter for {gamma}-Aminobutyric Acid in Arabidopsis thaliana
J. Biol. Chem.,
March 17, 2006;
281(11):
7197 - 7204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Wingler, S. Purdy, J. A. MacLean, and N. Pourtau
The role of sugars in integrating environmental signals during the regulation of leaf senescence
J. Exp. Bot.,
January 1, 2006;
57(2):
391 - 399.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Diaz, V. Saliba-Colombani, O. Loudet, P. Belluomo, L. Moreau, F. Daniel-Vedele, J.-F. Morot-Gaudry, and C. Masclaux-Daubresse
Leaf Yellowing and Anthocyanin Accumulation are Two Genetically Independent Strategies in Response to Nitrogen Limitation in Arabidopsis thaliana
Plant Cell Physiol.,
January 1, 2006;
47(1):
74 - 83.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
