First published online June 24, 2005; 10.1104/pp.105.062141
Plant Physiology 138:1596-1606 (2005)
© 2005 American Society of Plant Biologists
BIOENERGETICS AND PHOTOSYNTHESIS
In Vivo Respiratory Metabolism of Illuminated Leaves1
Guillaume Tcherkez*,
Gabriel Cornic,
Richard Bligny,
Elizabeth Gout and
Jaleh Ghashghaie
Laboratoire d'Ecophysiologie Végétale, Unité Mixte de Recherche 8079, Bât. 362, Centre scientifique d'Orsay, Université Paris XI, 91405 Orsay cedex, France (G.T., G.C., J.G.), and Laboratoire de Physiologie Cellulaire Végétale, Unité Mixte de Recherche 5168, Commissariat à l'Energie Atomique-Grenoble, 38054 Grenoble cedex 09, France (R.B., E.G.)
Day respiration of illuminated C3 leaves is not well understood and particularly, the metabolic origin of the day respiratory CO2 production is poorly known. This issue was addressed in leaves of French bean (Phaseolus vulgaris) using 12C/13C stable isotope techniques on illuminated leaves fed with 13C-enriched glucose or pyruvate. The 13CO2 production in light was measured using the deviation of the photosynthetic carbon isotope discrimination induced by the decarboxylation of the 13C-enriched compounds. Using different positional 13C-enrichments, it is shown that the Krebs cycle is reduced by 95% in the light and that the pyruvate dehydrogenase reaction is much less reduced, by 27% or less. Glucose molecules are scarcely metabolized to liberate CO2 in the light, simply suggesting that they can rarely enter glycolysis. Nuclear magnetic resonance analysis confirmed this view; when leaves are fed with 13C-glucose, leaf sucrose and glucose represent nearly 90% of the leaf 13C content, demonstrating that glucose is mainly directed to sucrose synthesis. Taken together, these data indicate that several metabolic down-regulations (glycolysis, Krebs cycle) accompany the light/dark transition and emphasize the decrease of the Krebs cycle decarboxylations as a metabolic basis of the light-dependent inhibition of mitochondrial respiration.
1 This work was supported by the European Community's Human Potential Programme (grant no. HPRN–CT–1999–00059, NEtwork for Terrestrial ecosystems CARbon Budget, to J.G.) and by the Centre National de la Recherche Scientifique (to G.C. and R.B.).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.062141.
* Corresponding author; e-mail guillaume.tcherkez{at}ese.u-psud.fr; fax 33–169153424.
Received March 2, 2005;
returned for revision April 4, 2005;
accepted April 10, 2005.
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