This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 139/2/779    most recent pp.105.068924v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Plant Physiol. Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Yamazoe, A. Articles by Nozaki, H. Search for Related Content PubMed PubMed Citation Articles by Yamazoe, A. Articles by Nozaki, H. Agricola Articles by Yamazoe, A. Articles by Nozaki, H.

DEVELOPMENT AND HORMONE ACTION

Characterization of Terfestatin A, a New Specific Inhibitor for Auxin Signaling^1,[w]

Department of Biochemistry, Okayama University of Science, Okayama City 700–0005, Japan (A.Y., K.-i.H., H.N.); and Department of Biology, University of York, York YO10 5YW, United Kingdom (S.K., O.L.)

Terfestatin A (TrfA), terphenyl--glucoside, was isolated from Streptomyces sp. F40 in a forward screen for compounds that inhibit the expression of auxin-inducible genes in Arabidopsis (Arabidopsis thaliana). TrfA specifically and competitively inhibited the expression of primary auxin-inducible genes in Arabidopsis roots, but did not affect the expression of genes regulated by other plant hormones such as abscisic acid and cytokinin. TrfA also blocked the auxin-enhanced degradation of auxin/indole-3-acetic acid (Aux/IAA) repressor proteins without affecting the auxin-stimulated interaction between Aux/IAAs and the F-box protein TIR1. TrfA treatment antagonized auxin responses in roots, including primary root inhibition, lateral root initiation, root hair promotion, and root gravitropism, but had only limited effects on shoot auxin responses. Taken together, these results indicate that TrfA acts as a modulator of Aux/IAA stability and thus provides a new tool for dissecting auxin signaling.

^[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.068924.

^* Corresponding author; e-mail hayashi{at}dbc.ous.ac.jp; fax 086–256–9556.

Received July 27, 2005; returned for revision August 9, 2005; accepted August 19, 2005.

Related articles in Plant Physiol.:

On the Inside

Peter V. Minorsky
Plant Physiol. 2005 139: 561-562. [Full Text]  

This article has been cited by other articles:

				P. E. Staswick The Tryptophan Conjugates of Jasmonic and Indole-3-Acetic Acids Are Endogenous Auxin Inhibitors Plant Physiology, July 1, 2009; 150(3): 1310 - 1321. [Abstract] [Full Text] [PDF]

				J. G. Dubrovsky, A. Soukup, S. Napsucialy-Mendivil, Z. Jeknic, and M. G. Ivanchenko The lateral root initiation index: an integrative measure of primordium formation Ann. Bot., March 1, 2009; 103(5): 807 - 817. [Abstract] [Full Text] [PDF]

				K.-i. Hayashi, X. Tan, N. Zheng, T. Hatate, Y. Kimura, S. Kepinski, and H. Nozaki Small-molecule agonists and antagonists of F-box protein-substrate interactions in auxin perception and signaling PNAS, April 8, 2008; 105(14): 5632 - 5637. [Abstract] [Full Text] [PDF]

				C. Sungur, S. Miller, J. Bergholz, R. C. Hoye, R. G. Brisbois, and P. Overvoorde The Small Molecule 2-Furylacrylic Acid Inhibits Auxin-Mediated Responses in Arabidopsis thaliana Plant Cell Physiol., December 1, 2007; 48(12): 1693 - 1701. [Abstract] [Full Text] [PDF]

				K. K. Biswas, C. Ooura, K. Higuchi, Y. Miyazaki, V. Van Nguyen, A. Rahman, H. Uchimiya, T. Kiyosue, T. Koshiba, A. Tanaka, et al. Genetic Characterization of Mutants Resistant to the Antiauxin p-Chlorophenoxyisobutyric Acid Reveals That AAR3, a Gene Encoding a DCN1-Like Protein, Regulates Responses to the Synthetic Auxin 2,4-Dichlorophenoxyacetic Acid in Arabidopsis Roots Plant Physiology, November 1, 2007; 145(3): 773 - 785. [Abstract] [Full Text] [PDF]

				K. Ruzicka, K. Ljung, S. Vanneste, R. Podhorska, T. Beeckman, J. Friml, and E. Benkova Ethylene Regulates Root Growth through Effects on Auxin Biosynthesis and Transport-Dependent Auxin Distribution PLANT CELL, July 1, 2007; 19(7): 2197 - 2212. [Abstract] [Full Text] [PDF]