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First published online April 7, 2006; 10.1104/pp.106.078444 Plant Physiology 141:404-411 (2006) © 2006 American Society of Plant Biologists
Nitric Oxide- and Hydrogen Peroxide-Responsive Gene Regulation during Cell Death Induction in Tobacco1,[W]Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B–9052 Ghent, Belgium (E.Z., S.M., J.F.D., P.A., D.I., F.V.B.); Dipartimento Scientifico e Tecnologico, Università degli Studi di Verona, I–37134 Verona, Italy (E.Z., A.F., M.D.); and Laboratoire de Biologie Environnementale, Université de Franche-Comté, Institut National de la Recherche Agronomique (EA3184), F–25030 Besançon cedex, France (J.F.D.)
Nitric oxide (NO) and hydrogen peroxide (H2O2) are regulatory molecules in various developmental processes and stress responses. Tobacco (Nicotiana tabacum) leaves exposed to moderate high light dramatically potentiated NO-mediated cell death in catalase-deficient (CAT1AS) but not in wild-type plants, providing genetic evidence for a partnership between NO and H2O2 during the induction of programmed cell death. With this experimental model system, the specific impact on gene expression was characterized by either NO or H2O2 alone or both molecules combined. By means of genome-wide cDNA-amplified fragment length polymorphism analysis, transcriptional changes were compared in high light-treated CAT1AS and wild-type leaves treated with or without the NO donor sodium nitroprusside. Differential gene expression was detected for 214 of the approximately 8,000 transcript fragments examined. For 108 fragments, sequence analysis revealed homology to genes with a role in signal transduction, defense response, hormone interplay, proteolysis, transport, and metabolism. Surprisingly, only 16 genes were specifically induced by the combined action of NO and H2O2, whereas the majority were regulated by either of them alone. At least seven transcription factors were mutually up-regulated, indicating significant overlap between NO and H2O2 signaling pathways. These results consolidate significant cross-talk between NO and H2O2, provide new insight into the early transcriptional response of plants to increased NO and H2O2 levels, and identify target genes of the combined action of NO and H2O2 during the induction of plant cell death.
1 This work was supported by grants from the European Union-Human Potential Program (HPMT–CT–2000–00088), the Research Fund of the Ghent University (Geconcerteerde Onderzoeksacties no. 12051403), the EMBO Young Investigator Program, the Research Fund of the University of Verona, the Vlaamse Gemeenschap (Tournesol T2005.18), and the Ministère des Affaires Etrangères (Programme d'Actions Intégrées Tournesol 08993ZL). 2 Present address: AlgoNomics N.V., Technologiepark 4, B–9052 Ghent, Belgium. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Frank Van Breusegem (frank.vanbreusegem{at}psb.ugent.be). [W] The online version of this article contains Web-only data. Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.106.078444. * Corresponding author; e-mail frank.vanbreusegem{at}psb.ugent.be; fax 32–9–3313809. Received January 31, 2006; returned for revision March 28, 2006; accepted March 29, 2006. This article has been cited by other articles:
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