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ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Trichomes of Tobacco Excrete Zinc as Zinc-Substituted Calcium Carbonate and Other Zinc-Containing Compounds^1,[W]

Environmental Geochemistry Group, Laboratoire de Géophysique Interne et Tectonophysique, University of Grenoble and Centre National de la Recherche Scientifique, 38041 Grenoble cedex 9, France (G.S., E.H., M.-P.I., N.G., A.M.); Division of Forest Resources, College of Forest Sciences, Kangwon National University, Chunchon 200–701, Kangwon-do, Korea (E.H., Y.-E.C.); Leibniz-Institut für Pflanzenbiochemie, 06120 Halle (Saale), Germany (E.H., M.B., S.C.); and Advanced Light Source, Berkeley Lab, MS 6–2100, Berkeley, California 94720 (S.F., M.A.M.)

Tobacco (Nicotiana tabacum L. cv Xanthi) plants were exposed to toxic levels of zinc (Zn). Zn exposure resulted in toxicity signs in plants, and these damages were partly reduced by a calcium (Ca) supplement. Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes. Exposure to Zn and Zn + Ca increased the trichome density and induced the production of Ca/Zn mineral grains on the head cells of trichomes. These grains were aggregates of submicrometer-sized crystals and poorly crystalline material and contained Ca as major element, along with subordinate amounts of Zn, manganese, potassium, chlorine, phosphorus, silicon, and magnesium. Micro x-ray diffraction revealed that the large majority of the grains were composed essentially of metal-substituted calcite (CaCO₃). CaCO₃ polymorphs (aragonite and vaterite) and CaC₂O₄ (Ca oxalate) mono- and dihydrate also were identified, either as an admixture to calcite or in separate grains. Some grains did not diffract, although they contained Ca, suggesting the presence of amorphous form of Ca. The presence of Zn-substituted calcite was confirmed by Zn K-edge micro-extended x-ray absorption fine structure spectroscopy. Zn bound to organic compounds and Zn-containing silica and phosphate were also identified by this technique. The proportion of Zn-substituted calcite relative to the other species increased with Ca exposure. The production of Zn-containing biogenic calcite and other Zn compounds through the trichomes is a novel mechanism involved in Zn detoxification. This study illustrates the potential of laterally resolved x-ray synchrotron radiation techniques to study biomineralization and metal homeostasis processes in plants.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Yong-Eui Choi (yechoi{at}kangwon.ac.kr).

^[W] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.106.082743.

^* Corresponding author; e-mail yechoi{at}kangwon.ac.kr; fax 82–33–252–8310.

Received April 28, 2006; returned for revision April 28, 2006; accepted May 22, 2006.

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