This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 141/4/1555    most recent pp.106.080218v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Zalejski, C. Articles by Jeannette, E. Search for Related Content PubMed PubMed Citation Articles by Zalejski, C. Articles by Jeannette, E. Agricola Articles by Zalejski, C. Articles by Jeannette, E.

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Induction of Abscisic Acid-Regulated Gene Expression by Diacylglycerol Pyrophosphate Involves Ca²⁺ and Anion Currents in Arabidopsis Suspension Cells^1,[W]

Université Pierre et Marie Curie-Paris 6 and Centre National de la Recherche Scientifique, FRE 2846, Physiologie Cellulaire et Moléculaire des Plantes, F–94200 Ivry-sur-Seine, France (C.Z., S.P., R.M., Y.H., E.M., E.J.); and Université Paris 7-Denis Diderot, Equipe d'Accueil 3514, Electrophysiologie des Membranes, F–75251 Paris cedex 05, France (J.-P.R.)

Diacylglycerol pyrophosphate (DGPP) was recently shown to be a possible intermediate in abscisic acid (ABA) signaling. In this study, reverse transcription-PCR of ABA up-regulated genes was used to evaluate the ability of DGPP to trigger gene expression in Arabidopsis (Arabidopsis thaliana) suspension cells. At5g06760, LTI30, RD29A, and RAB18 were stimulated by ABA and also specifically expressed in DGPP-treated cells. Use of the Ca²⁺ channel blockers fluspirilene and pimozide and the Ca²⁺ chelator EGTA showed that Ca²⁺ was required for ABA induction of DGPP formation. In addition, Ca²⁺ participated in DGPP induction of gene expression via stimulation of anion currents. Hence, a sequence of Ca²⁺, DGPP, and anion currents, constituting a core of early ABA-signaling events necessary for gene expression, is proposed.

² These authors contributed equally to the paper.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Emmanuelle Jeannette (ema{at}ccr.jussieu.fr).

^[W] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.106.080218.

^* Corresponding author; e-mail ema{at}ccr.jussieu.fr; fax 33–1–44–27–62–32.

Received March 16, 2006; returned for revision May 2, 2006; accepted May 23, 2006.

This article has been cited by other articles:

				E. Darwish, C. Testerink, M. Khalil, O. El-Shihy, and T. Munnik Phospholipid Signaling Responses in Salt-Stressed Rice Leaves Plant Cell Physiol., May 1, 2009; 50(5): 986 - 997. [Abstract] [Full Text] [PDF]

				J. Trouverie, G. Vidal, Z. Zhang, C. Sirichandra, K. Madiona, Z. Amiar, J.-L. Prioul, E. Jeannette, J.-P. Rona, and M. Brault Anion Channel Activation and Proton Pumping Inhibition Involved in the Plasma Membrane Depolarization Induced by ABA in Arabidopsis thaliana Suspension Cells are Both ROS Dependent Plant Cell Physiol., October 1, 2008; 49(10): 1495 - 1507. [Abstract] [Full Text] [PDF]