OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 144/2/637    most recent pp.106.087437v2 pp.106.087437v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Peumans, W. J. Articles by Van Damme, E. J.M. Search for Related Content PubMed PubMed Citation Articles by Peumans, W. J. Articles by Van Damme, E. J.M. Agricola Articles by Peumans, W. J. Articles by Van Damme, E. J.M. Related Collections Legume Biology

The Liverwort Marchantia polymorpha Expresses Orthologs of the Fungal Agaricus bisporus Agglutinin Family^1,[W],[OA]

Nievelveldweg 9, B–9310 Aalst, Belgium (W.J.P.); Department of Molecular Biotechnology, Laboratory of Biochemistry and Glycobiology (E.F., N.L., E.J.M.V.D.) and Laboratory for Protein Biochemistry and Protein Engineering (B.D.), Ghent University, 9000 Ghent, Belgium; l'Institut Fédératif de Recherche 40-Centre National de la Recherche Scientifique, Signalisation et Biotechnologie Végétales, 31326 Castanet Tolosan, France (A.J.); Surfaces Cellulaires et Signalisation chez les Végétaux, Unité Mixte de la Recherche, Centre National de la Recherche Scientifique-Université Paul Sabatier 5546, Pôle de Biotechnologies Végétales, 31326 Castanet-Tolosan, France (P.R.); Department of Applied Science, Okayama University of Science, Okayama 700–0005, Japan (H.H.); and Department of Biochemistry and Molecular Biology, University of Oklahoma, Health Sciences Center, Oklahoma City, Oklahoma (R.A.)

A lectin different from the previously described mannose-binding agglutinins has been isolated from the liverwort Marchantia polymorpha. Biochemical characterization of the purified lectin combined with the data from earlier transcriptome analyses demonstrated that the novel M. polymorpha agglutinin is not related to any of the known plant lectin families, but closely resembles the Agaricus bisporus-type lectins, which hitherto have been found exclusively in fungi. Immunolocalization studies confirmed that lectin is exclusively associated with plant cells, ruling out the possibility of a fungal origin. Extensive screening of publicly accessible databases confirmed that, apart from fungi, the occurrence of A. bisporus-type lectins is confined to M. polymorpha and the moss Tortula ruralis. Expression of a typical fungal protein in a liverwort and a moss raises the question of the origin of the corresponding genes. Regardless of the evolutionary origin, the presence of a functional A. bisporus lectin ortholog in M. polymorpha provides evidence for the expression of an additional carbohydrate-binding domain in Viridiplantae.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instruction for Authors (www.plantphysiol.org) is: Els J.M. Van Damme (elsjm.vandamme{at}ugent.be).

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.106.087437

^* Corresponding author; e-mail elsjm.vandamme{at}ugent.be; fax 32–92646219.

Received July 27, 2006; accepted September 24, 2006; published October 13, 2006.

This article has been cited by other articles:

				E. Fouquaert, W. J. Peumans, D. F. Smith, P. Proost, S. N. Savvides, and E. J.M. Van Damme The "Old" Euonymus europaeus Agglutinin Represents a Novel Family of Ubiquitous Plant Proteins Plant Physiology, July 1, 2008; 147(3): 1316 - 1324. [Abstract] [Full Text] [PDF]

				S. Subramanyam, D. F. Smith, J. C. Clemens, M. A. Webb, N. Sardesai, and C. E. Williams Functional Characterization of HFR1, a High-Mannose N-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae Plant Physiology, July 1, 2008; 147(3): 1412 - 1426. [Abstract] [Full Text] [PDF]