OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 146/3/1219    most recent pp.107.110742v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Tamaoki, M. Articles by Pilon-Smits, E. A.H. Search for Related Content PubMed PubMed Citation Articles by Tamaoki, M. Articles by Pilon-Smits, E. A.H. Agricola Articles by Tamaoki, M. Articles by Pilon-Smits, E. A.H. Related Collections Reactive Oxygen Species

ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Cooperative Ethylene and Jasmonic Acid Signaling Regulates Selenite Resistance in Arabidopsis^1,[W],[OA]

Biology Department, Colorado State University, Fort Collins, Colorado 80523 (M.T., J.L.F., E.A.H.P.-S.); Environmental Biology Division, National Institute for Environmental Studies, Tsukuba, Ibaraki 305–8506, Japan (M.T.); and Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305–8577, Japan (M.T.)

Selenium (Se) is an essential element for many organisms, but excess Se is toxic. To better understand plant Se toxicity and resistance mechanisms, we compared the physiological and molecular responses of two Arabidopsis (Arabidopsis thaliana) accessions, Columbia (Col)-0 and Wassilewskija (Ws)-2, to selenite treatment. Measurement of root length Se tolerance index demonstrated a clear difference between selenite-resistant Col-0 and selenite-sensitive Ws-2. Macroarray analysis showed more pronounced selenite-induced increases in mRNA levels of ethylene- or jasmonic acid (JA)-biosynthesis and -inducible genes in Col-0 than in Ws-2. Indeed, Col-0 exhibited higher levels of ethylene and JA. The selenite-sensitive phenotype of Ws-2 was attenuated by treatment with ethylene precursor or methyl jasmonate (MeJA). Conversely, the selenite resistance of Col-0 was reduced in mutants impaired in ethylene or JA biosynthesis or signaling. Genes encoding sulfur (S) transporters and S assimilation enzymes were up-regulated by selenite in Col-0 but not Ws-2. Accordingly, Col-0 contained higher levels of total S and Se and of nonprotein thiols than Ws-2. Glutathione redox status was reduced by selenite in Ws-2 but not in Col-0. Furthermore, the generation of reactive oxygen species by selenite was higher in Col-0 than in Ws-2. Together, these results indicate that JA and ethylene play important roles in Se resistance in Arabidopsis. Reactive oxygen species may also have a signaling role, and the resistance mechanism appears to involve enhanced S uptake and reduction.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Masanori Tamaoki (mtamaoki{at}nies.go.jp).

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.110742

^* Corresponding author; e-mail mtamaoki{at}nies.go.jp.

Received October 10, 2007; accepted December 22, 2007; published January 4, 2008.

This article has been cited by other articles:

				C. M. Cohu, S. E. Abdel-Ghany, K. A. Gogolin Reynolds, A. M. Onofrio, J. R. Bodecker, J. A. Kimbrel, K. K. Niyogi, and M. Pilon Copper Delivery by the Copper Chaperone for Chloroplast and Cytosolic Copper/Zinc-Superoxide Dismutases: Regulation and Unexpected Phenotypes in an Arabidopsis Mutant Mol Plant, November 1, 2009; 2(6): 1336 - 1350. [Abstract] [Full Text] [PDF]

				X. Zhou, Y. Yuan, Y. Yang, M. Rutzke, T. W. Thannhauser, L. V. Kochian, and L. Li Involvement of a Broccoli COQ5 Methyltransferase in the Production of Volatile Selenium Compounds Plant Physiology, October 1, 2009; 151(2): 528 - 540. [Abstract] [Full Text] [PDF]

				V. Hugouvieux, C. Dutilleul, A. Jourdain, F. Reynaud, V. Lopez, and J. Bourguignon Arabidopsis Putative Selenium-Binding Protein1 Expression Is Tightly Linked to Cellular Sulfur Demand and Can Reduce Sensitivity to Stresses Requiring Glutathione for Tolerance Plant Physiology, October 1, 2009; 151(2): 768 - 781. [Abstract] [Full Text] [PDF]