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BIOENERGETICS AND PHOTOSYNTHESIS

Expression Analysis of Genes Associated with the Induction of the Carbon-Concentrating Mechanism in Chlamydomonas reinhardtii^1,[W],[OA]

Graduate School of Biostudies, Kyoto University, Kyoto 606–8502, Japan

Acclimation to varying CO₂ concentrations and light intensities is associated with the monitoring of environmental changes by controlling genetic and physiological responses through CO₂ and light signal transduction. While CO₂ and light signals are indispensable for photosynthesis, and these environmental factors have been proposed as strongly associated with each other, studies linking these components are largely limited to work on higher plants. In this study, we examined the physiological characteristics of a green alga, Chlamydomonas reinhardtii, exposed to various light intensities or CO₂ concentrations. Acclimation to CO₂-limiting conditions by Chlamydomonas requires the induction of a carbon-concentrating mechanism (CCM) to allow the uptake of inorganic carbon (Ci) and increase the affinity for Ci. We revealed that the induction of the CCM is not solely dependent on absolute environmental Ci concentrations but is also affected by light intensity. Using a cDNA array containing 10,368 expressed sequence tags, we also obtained global expression profiles related to the physiological responses. The induction of several CCM-associated genes was strongly affected by high light as well as CO₂ concentrations. We identified novel candidates for Ci transporters and CO₂-responsive regulatory factors whose expression levels were significantly increased during the induction of the CCM.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Hideya Fukuzawa (fukuzawa{at}lif.kyoto-u.ac.jp).

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.114652

^* Corresponding author; e-mail fukuzawa{at}lif.kyoto-u.ac.jp.

Received December 6, 2007; accepted February 24, 2008; published March 5, 2008.