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Genetic Evidence That Cellulose Synthase Activity Influences Microtubule Cortical Array Organization^1,[W],[OA]

Department of Plant Biology, Carnegie Institution, Stanford, California 94305 (A.R.P., D.W.E., C.R.S.); Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 (A.R.P.); Department of Biological Sciences, Stanford University, Stanford, California 94305 (A.R.P., C.R.S.); and Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14476 Golm, Germany (S.P.)

To identify factors that influence cytoskeletal organization we screened for Arabidopsis (Arabidopsis thaliana) mutants that show hypersensitivity to the microtubule destabilizing drug oryzalin. We cloned the genes corresponding to two of the 131 mutant lines obtained. The genes encoded mutant alleles of PROCUSTE1 and KORRIGAN, which both encode proteins that have previously been implicated in cellulose synthesis. Analysis of microtubules in the mutants revealed that both mutants have altered orientation of root cortical microtubules. Similarly, isoxaben, an inhibitor of cellulose synthesis, also altered the orientation of cortical microtubules while exogenous cellulose degradation did not. Thus, our results substantiate that proteins involved in cell wall biosynthesis influence cytoskeletal organization and indicate that this influence on cortical microtubule stability and orientation is correlated with cellulose synthesis rather than the integrity of the cell wall.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Chris R. Somerville (crs{at}berkeley.edu).

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www.plantphysiol.org/cgi/doi/10.1104/pp.108.120196

^* Corresponding author; e-mail crs{at}berkeley.edu.

Received April 2, 2008; accepted June 22, 2008; published June 26, 2008.

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