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The PRA1 Gene Family in Arabidopsis^1,[W]

Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941–590, Brazil (C.L.A.K.); and Department of Plant Systems Biology, Flanders Institute for Biotechnology (C.L.A.K., J.B., K.V., H.V.d.D., S.M., E.R., D.I., L.D.V.), and Department of Molecular Genetics, Ghent University, B–9052 Gent, Belgium (C.L.A.K., J.B., K.V., H.V.d.D., S.M., E.R., D.I., L.D.V.)

Prenylated Rab acceptor 1 (PRA1) domain proteins are small transmembrane proteins that regulate vesicle trafficking as receptors of Rab GTPases and the vacuolar soluble N-ethylmaleimide-sensitive factor attachment receptor protein VAMP2. However, little is known about PRA1 family members in plants. Sequence analysis revealed that higher plants, compared with animals and primitive plants, possess an expanded family of PRA1 domain-containing proteins. The Arabidopsis (Arabidopsis thaliana) PRA1 (AtPRA1) proteins were found to homodimerize and heterodimerize in a manner corresponding to their phylogenetic distribution. Different AtPRA1 family members displayed distinct expression patterns, with a preference for vascular cells and expanding or developing tissues. AtPRA1 genes were significantly coexpressed with Rab GTPases and genes encoding vesicle transport proteins, suggesting an involvement in the vesicle trafficking process similar to that of their animal counterparts. Correspondingly, AtPRA1 proteins were localized in the endoplasmic reticulum, Golgi apparatus, and endosomes/prevacuolar compartments, hinting at a function in both secretory and endocytic intracellular trafficking pathways. Taken together, our data reveal a high functional diversity of AtPRA1 proteins, probably dealing with the various demands of the complex trafficking system.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Lieven De Veylder (lieven.deveylder{at}psb.ugent.be).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.122226

^* Corresponding author; e-mail dirk.inze{at}psb.ugent.be.

Received April 29, 2008; accepted June 25, 2008; published June 26, 2008.

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