OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 147/4/1984    most recent pp.108.116632v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Endo, A. Articles by Nambara, E. Search for Related Content PubMed PubMed Citation Articles by Endo, A. Articles by Nambara, E. Agricola Articles by Endo, A. Articles by Nambara, E.

ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Drought Induction of Arabidopsis 9-cis-Epoxycarotenoid Dioxygenase Occurs in Vascular Parenchyma Cells^1,[W],[OA]

Department of Biological Sciences, Tokyo Metropolitan University, Hachiouji, Tokyo 192–0397, Japan (A.E., M.O., K.I., H.K., M.S., T.K.); Growth Regulation Research Group, RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa 230–0045, Japan (A.E., M.O., Y.K., E.N.); Course of the Science of Bioresource, United Graduate School of Agricultural Science, Iwate University, Morioka, Iwate 020–8550, Japan (Y.S., T.T., W.M.); Department of Bioresource Engineering, Yamagata University, Tsuruoka, Yamagata 997–8555, Japan (T.T., W.M.); Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113–8657, Japan (H.T., M.N.); and Gene Discovery Research Group, RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa 230–0045, Japan (K.S.)

The regulation of abscisic acid (ABA) biosynthesis is essential for plant responses to drought stress. In this study, we examined the tissue-specific localization of ABA biosynthetic enzymes in turgid and dehydrated Arabidopsis (Arabidopsis thaliana) plants using specific antibodies against 9-cis-epoxycarotenoid dioxygenase 3 (AtNCED3), AtABA2, and Arabidopsis aldehyde oxidase 3 (AAO3). Immunohistochemical analysis revealed that in turgid plants, AtABA2 and AAO3 proteins were localized in vascular parenchyma cells most abundantly at the boundary between xylem and phloem bundles, but the AtNCED3 protein was undetectable in these tissues. In water-stressed plants, AtNCED3 was detected exclusively in the vascular parenchyma cells together with AtABA2 and AAO3. In situ hybridization using the antisense probe for AtNCED3 showed that the drought-induced expression of AtNCED3 was also restricted to the vascular tissues. Expression analysis of laser-microdissected cells revealed that, among nine drought-inducible genes examined, the early induction of most genes was spatially restricted to vascular cells at 1 h and then some spread to mesophyll cells at 3 h. The spatial constraint of AtNCED3 expression in vascular tissues provides a novel insight into plant systemic response to drought stresses.

² Present address: Department of Cell and Systems Biology, University of Toronto, 25 Willcocks St., Toronto, Ontario, Canada M5S 3B2.

³ Present address: Plant Functional Genomics Research Group, RIKEN Plant Science Center, Suehiro-cho 1–7–22, Tsurumi, Yokohama, Kanagawa 230–0045, Japan.

⁴ Present address: National Institute of Landstock and Grassland Science, Senbonmatsu, Nasushiobara, Tochigi 329–2793, Japan.

⁵ Present address: Dormancy and Adaptation Research Unit, RIKEN Plant Science Center, 1–7–22, Suehiro-cho, Tsurumi, Yokohama 230–0045, Japan.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Eiji Nambara (eiji.nambara{at}utoronto.ca).

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.116632

^* Corresponding author; e-mail eiji.nambara{at}utoronto.ca.

Received January 21, 2008; accepted May 27, 2008; published June 11, 2008.

This article has been cited by other articles:

				J. Wan, R. Griffiths, J. Ying, P. McCourt, and Y. Huang Development of Drought-Tolerant Canola (Brassica napus L.) through Genetic Modulation of ABA-mediated Stomatal Responses Crop Sci., August 7, 2009; 49(5): 1539 - 1554. [Abstract] [Full Text] [PDF]

				G. Galvez-Valdivieso, M. J. Fryer, T. Lawson, K. Slattery, W. Truman, N. Smirnoff, T. Asami, W. J. Davies, A. M. Jones, N. R. Baker, et al. The High Light Response in Arabidopsis Involves ABA Signaling between Vascular and Bundle Sheath Cells PLANT CELL, July 1, 2009; 21(7): 2143 - 2162. [Abstract] [Full Text] [PDF]

				D. Konopka-Postupolska, G. Clark, G. Goch, J. Debski, K. Floras, A. Cantero, B. Fijolek, S. Roux, and J. Hennig The Role of Annexin 1 in Drought Stress in Arabidopsis Plant Physiology, July 1, 2009; 150(3): 1394 - 1410. [Abstract] [Full Text] [PDF]

				B. Parent, C. Hachez, E. Redondo, T. Simonneau, F. Chaumont, and F. Tardieu Drought and Abscisic Acid Effects on Aquaporin Content Translate into Changes in Hydraulic Conductivity and Leaf Growth Rate: A Trans-Scale Approach Plant Physiology, April 1, 2009; 149(4): 2000 - 2012. [Abstract] [Full Text] [PDF]

				M. Okamoto, Y. Tanaka, S. R. Abrams, Y. Kamiya, M. Seki, and E. Nambara High Humidity Induces Abscisic Acid 8'-Hydroxylase in Stomata and Vasculature to Regulate Local and Systemic Abscisic Acid Responses in Arabidopsis Plant Physiology, February 1, 2009; 149(2): 825 - 834. [Abstract] [Full Text] [PDF]