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DEVELOPMENT AND HORMONE ACTION

Proteome-Wide Characterization of Seed Aging in Arabidopsis: A Comparison between Artificial and Natural Aging Protocols^[W],[OA]

UMR 204, INRA-AgroParisTech, Laboratoire de Biologie des Semences, AgroParisTech, Chaire de Physiologie Végétale, F–75231 Paris cedex 05, France (L.R.); UMR 204, INRA-AgroParisTech, Laboratoire de Biologie des Semences, Institut Jean-Pierre Bourgin-INRA, F–78026 Versailles cedex, France (L.R.); UMR 5240, CNRS-Université Claude Bernard Lyon-Institut National des Sciences Appliquées-Bayer CropScience Joint Laboratory, Bayer CropScience, F–69263 Lyon cedex 9, France (Y.L., C.J., D.J.); Plant Research International, Wageningen University and Research Center, 6700 AA Wageningen, The Netherlands (S.P.C.G.); and Centre d'Analyse Protéomique de Marseille, Institut Fédératif de Recherche Jean Roche, F–13916 Marseille cedex 20, France (M.B.)

A variety of mechanisms have been proposed to account for the extension of life span in seeds (seed longevity). In this work, we used Arabidopsis (Arabidopsis thaliana) seeds as a model and carried out differential proteomics to investigate this trait, which is of both ecological and agricultural importance. In our system based on a controlled deterioration treatment (CDT), we compared seed samples treated for different periods of time up to 7 d. Germination tests showed a progressive decrease of germination vigor depending on the duration of CDT. Proteomic analyses revealed that this loss in seed vigor can be accounted for by protein changes in the dry seeds and by an inability of the low-vigor seeds to display a normal proteome during germination. Furthermore, CDT strongly increased the extent of protein oxidation (carbonylation), which might induce a loss of functional properties of seed proteins and enzymes and/or enhance their susceptibility toward proteolysis. These results revealed essential mechanisms for seed vigor, such as translational capacity, mobilization of seed storage reserves, and detoxification efficiency. Finally, this work shows that similar molecular events accompany artificial and natural seed aging.

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www.plantphysiol.org/cgi/doi/10.1104/pp.108.123141

^* Corresponding author; e-mail loic.rajjou{at}agroparistech.fr.

Received May 19, 2008; accepted June 20, 2008; published July 3, 2008.

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