OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) PPT slides of all figures Supplemental Data OA All Versions of this Article: 148/2/908    most recent pp.108.125633v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Xu, S. B. Articles by Wang, T. Search for Related Content PubMed PubMed Citation Articles by Xu, S. B. Articles by Wang, T. Agricola Articles by Xu, S. B. Articles by Wang, T.

DEVELOPMENT AND HORMONE ACTION

Dynamic Proteomic Analysis Reveals a Switch between Central Carbon Metabolism and Alcoholic Fermentation in Rice Filling Grains^1,[W],[OA]

Research Center of Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (S.B.X., T.L., Z.Y.D., K.C., T.W.); Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Beijing 10010, China (Y.X.); National Center for Plant Gene Research, Beijing 100093, China (K.C., T.W.); and Graduate School of Chinese Academy of Sciences, Beijing 100049, China (S.B.X., T.L.)

Accumulation of reserve materials in filling grains involves the coordination of different metabolic and cellular processes, and understanding the molecular mechanisms underlying the interconnections remains a major challenge for proteomics. Rice (Oryza sativa) is an excellent model for studying grain filling because of its importance as a staple food and the available genome sequence database. Our observations showed that embryo differentiation and endosperm cellularization in developing rice seeds were completed approximately 6 d after flowering (DAF); thereafter, the immature seeds mainly underwent cell enlargement and reached the size of mature seeds at 12 DAF. Grain filling began at 6 DAF and lasted until 20 DAF. Dynamic proteomic analyses revealed 396 protein spots differentially expressed throughout eight sequential developmental stages from 6 to 20 DAF and determined 345 identities. These proteins were involved in different cellular and metabolic processes with a prominently functional skew toward metabolism (45%) and protein synthesis/destination (20%). Expression analyses of protein groups associated with different functional categories/subcategories showed that substantially up-regulated proteins were involved in starch synthesis and alcoholic fermentation, whereas the down-regulated proteins in the process were involved in central carbon metabolism and most of the other functional categories/subcategories such as cell growth/division, protein synthesis, proteolysis, and signal transduction. The coordinated changes were consistent with the transition from cell growth and differentiation to starch synthesis and clearly indicated that a switch from central carbon metabolism to alcoholic fermentation may be important for starch synthesis and accumulation in the developmental process.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Tai Wang (twang{at}ibcas.ac.cn).

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.125633

^* Corresponding author; e-mail twang{at}ibcas.ac.cn.

Received July 1, 2008; accepted August 25, 2008; published August 27, 2008.

This article has been cited by other articles:

				M.-S. Yun and Y. Kawagoe Amyloplast Division Progresses Simultaneously at Multiple Sites in the Endosperm of Rice Plant Cell Physiol., September 1, 2009; 50(9): 1617 - 1626. [Abstract] [Full Text] [PDF]