Plant Physiology Preview
Published on April 19, 2002; 10.1104/pp.000869
Received February 17, 2002
Accepted February 17, 2002
LeCPK1, a Calcium-Dependent Protein Kinase from
Tomato. Plasma Membrane Targeting and Biochemical
Characterization
Frank Rutschmann , Urs Stalder , Markus Piotrowski , Claudia Oecking , and Andreas Schaller *
Institute of Plant Sciences, Plant Biochemistry and Physiology Group, Swiss Federal Institute of Technology, Universitätstrasse 2, CH--8092 Zürich, Switzerland (F.R., U.S., A.S.); Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, D--44780 Bochum, Germany (M.P.); and Center for Plant Molecular Biology (ZMBP), Universität Tübingen, Auf der Morgenstelle 5, D--72076 Tübingen, Germany (C.O.)
* Corresponding author; email: andreas.schaller{at}ipw.biol.ethz.ch.
The cDNA of LeCPK1, a calcium-dependent protein kinase, was cloned from tomato (Lycopersicon esculentum Mill.). LeCPK1 was expressed in Escherichia coli and purified from bacterial extracts. The recombinant protein was shown to be a functional protein kinase using a synthetic peptide as the substrate (syntide-2, Km = 85 µM). Autophosphorylation of LeCPK1 was observed on threonine and serine residues, one of which was identified as serine-439. Kinase activity was shown to be Ca2+ dependent and required the C-terminal, calmodulin-like domain of LeCPK1. Two classes of high- and low-affinity Ca2+-binding sites were observed, exhibiting dissociation constants of 0.6 and 55 µM, respectively. LeCPK1 was found to phosphorylate the regulatory C-terminal domain of the plasma membrane H+-ATPase in vitro. A potential role in the regulation of proton pump activity is corroborated by the apparent colocalization of the plasma membrane H+-ATPase and LeCPK1 in vivo. Upon transient expression in suspension-cultured cells, a C-terminal fusion of LeCPK1 with the green fluorescent protein was targeted to the plasma membrane. Myristoylation of the LeCPK1 N terminus was found to be required for plasma membrane targeting.
This article has been cited by other articles:
|
|
|
|
 
P. K. Kandoth, S. Ranf, S. S. Pancholi, S. Jayanty, M. D. Walla, W. Miller, G. A. Howe, D. E. Lincoln, and J. W. Stratmann
Tomato MAPKs LeMPK1, LeMPK2, and LeMPK3 function in the systemin-mediated defense response against herbivorous insects
PNAS,
July 17, 2007;
104(29):
12205 - 12210.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. T. Fuglsang, Y. Guo, T. A. Cuin, Q. Qiu, C. Song, K. A. Kristiansen, K. Bych, A. Schulz, S. Shabala, K. S. Schumaker, et al.
Arabidopsis Protein Kinase PKS5 Inhibits the Plasma Membrane H+-ATPase by Preventing Interaction with 14-3-3 Protein
PLANT CELL,
May 1, 2007;
19(5):
1617 - 1634.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kobayashi, I. Ohura, K. Kawakita, N. Yokota, M. Fujiwara, K. Shimamoto, N. Doke, and H. Yoshioka
Calcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH Oxidase
PLANT CELL,
March 1, 2007;
19(3):
1065 - 1080.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-C. Yu, M.-J. Li, G.-F. Gao, H.-Z. Feng, X.-Q. Geng, C.-C. Peng, S.-Y. Zhu, X.-J. Wang, Y.-Y. Shen, and D.-P. Zhang
Abscisic Acid Stimulates a Calcium-Dependent Protein Kinase in Grape Berry
Plant Physiology,
February 1, 2006;
140(2):
558 - 579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D. Weyman, Z. Pan, Q. Feng, D. G. Gilchrist, and R. M. Bostock
A Circadian Rhythm-Regulated Tomato Gene Is Induced by Arachidonic Acid and Phythophthora infestans Infection
Plant Physiology,
January 1, 2006;
140(1):
235 - 248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Szczegielniak, M. Klimecka, A. Liwosz, A. Ciesielski, S. Kaczanowski, G. Dobrowolska, A. C. Harmon, and G. Muszynska
A Wound-Responsive and Phospholipid-Regulated Maize Calcium-Dependent Protein Kinase
Plant Physiology,
December 1, 2005;
139(4):
1970 - 1983.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Leclercq, B. Ranty, M.-T. Sanchez-Ballesta, Z. Li, B. Jones, A. Jauneau, J.-C. Pech, A. Latche, R. Ranjeva, and M. Bouzayen
Molecular and biochemical characterization of LeCRK1, a ripening-associated tomato CDPK-related kinase
J. Exp. Bot.,
January 1, 2005;
56(409):
25 - 35.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Christodoulou, A. Malmendal, J. F. Harper, and W. J. Chazin
Evidence for Differing Roles for Each Lobe of the Calmodulin-like Domain in a Calcium-dependent Protein Kinase
J. Biol. Chem.,
July 9, 2004;
279(28):
29092 - 29100.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. W. Chehab, O. R. Patharkar, A. D. Hegeman, T. Taybi, and J. C. Cushman
Autophosphorylation and Subcellular Localization Dynamics of a Salt- and Water Deficit-Induced Calcium-Dependent Protein Kinase from Ice Plant
Plant Physiology,
July 1, 2004;
135(3):
1430 - 1446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Ludwig, T. Romeis, and J. D. G. Jones
CDPK-mediated signalling pathways: specificity and cross-talk
J. Exp. Bot.,
January 2, 2004;
55(395):
181 - 188.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Dammann, A. Ichida, B. Hong, S. M. Romanowsky, E. M. Hrabak, A. C. Harmon, B. G. Pickard, and J. F. Harper
Subcellular Targeting of Nine Calcium-Dependent Protein Kinase Isoforms from Arabidopsis
Plant Physiology,
August 1, 2003;
132(4):
1840 - 1848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Hrabak, C. W.M. Chan, M. Gribskov, J. F. Harper, J. H. Choi, N. Halford, J. Kudla, S. Luan, H. G. Nimmo, M. R. Sussman, et al.
The Arabidopsis CDPK-SnRK Superfamily of Protein Kinases
Plant Physiology,
June 1, 2003;
132(2):
666 - 680.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
