Plant Physiology Preview
Published on July 25, 2002; 10.1104/pp.001636
Received December 12, 2001
Returned for revision February 11, 2002
Accepted April 16, 2002
In Vivo Interactions between Photosynthesis, Mitorespiration, and Chlororespiration in Chlamydomonas reinhardtii
Laurent Cournac *, Gwendal Latouche , Zoran Cerovic , Kevin Redding , Jacques Ravenel , and Gilles Peltier
Commissariat à l'Energie Atomique (CEA) Cadarache, Direction des Sciences du Vivant, Département d'Ecophysiologie Végétale et de Microbiologie, Laboratoire d'Ecophysiologie de la Photosynthèse, Unité Mixte de Recherche 163 Centre National de la Recherche Scientifique CEA, Univ-Méditerranée CEA 1000, F--13108 Saint-Paul-lez-Durance cedex, France (L.C., J.R., G.P.); Laboratoire pour l'Utilisation du Rayonnement Electromagnétique-Centre National de la Recherche Scientifique, Bat 203, Boîte Postale 34, Centre Universitaire Paris-Sud, Equipe Photosynthèse et Télédétection, F--91898 Orsay, France (G.L., Z.C.); and Department of Chemistry, 120 Lloyd Hall, 6th Avenue, University of Alabama, Tuscaloosa, Alabama 35487--0336 (K.R.)
* Corresponding author; email: laurent.cournac{at}cea.fr.
Interactions between photosynthesis, mitochondrial respiration (mitorespiration), and chlororespiration have been investigated in the green alga Chlamydomonas reinhardtii using flash illumination and a bare platinum electrode. Depending on the physiological status of algae, flash illumination was found to induce either a fast (t1/2  300 ms) or slow (t1/2 3 s) transient inhibition of oxygen uptake. Based on the effects of the mitorespiratory inhibitors myxothiazol and salicyl hydroxamic acid (SHAM), and of propyl gallate, an inhibitor of the chlororespiratory oxidase, we conclude that the fast transient is due to the flash-induced inhibition of chlororespiration and that the slow transient is due to the flash-induced inhibition of mitorespiration. By measuring blue-green fluorescence changes, related to the redox status of the pyridine nucleotide pool, and chlorophyll fluorescence, related to the redox status of plastoquinones (PQs) in C. reinhardtii wild type and in a photosystem I-deficient mutant, we show that interactions between photosynthesis and chlororespiration are favored when PQ and pyridine nucleotide pools are reduced, whereas interactions between photosynthesis and mitorespiration are favored at more oxidized states. We conclude that the plastid oxidase, similar to the mitochondrial alternative oxidase, becomes significantly engaged when the PQ pool becomes highly reduced, and thereby prevents its over-reduction.
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