Plant Physiology Preview
Published on June 14, 2002; 10.1104/pp.003558
Received February 1, 2002
Returned for revision March 2, 2002
Accepted March 26, 2002
Novel Insight into Vascular, Stress, and Auxin-Dependent and
-Independent Gene Expression Programs in Strawberry, a Non-Climacteric
Fruit
Asaph Aharoni *, Leopold C.P. Keizer , Hetty C. Van Den Broeck , Rosario Blanco-Portales , Juan Muñoz-Blanco , Gregory Bois , Patrick Smit , Ric C.H. De Vos , and Ann P. O'Connell
Business Unit Cell Cybernetics(A.A., H.C.V.D.B., G.B., P.S., R.C.H.D.V., A.P.O.) and Business Unit Biometry (L.C.P.K.), Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands; and Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, 14071 Córdoba, Spain (R.B.-P., J.M.-B.)
* Corresponding author; email: a.aharoni{at}plant.wag-ur.nl.
Using cDNA microarrays, a comprehensive investigation of gene expression was carried out in strawberry (Fragaria x ananassa) fruit to understand the flow of events associated with its maturation and non-climacteric ripening. We detected key processes and novel genes not previously associated with fruit development and ripening, related to vascular development, oxidative stress, and auxin response. Microarray analysis during fruit development and in receptacle and seed (achene) tissues established an interesting parallelism in gene expression between the transdifferentiation of tracheary elements in Zinnia elegans and strawberry. One of the genes, CAD, common to both systems and encoding the lignin-related protein cinnamyl alcohol dehydrogenase, was immunolocalized to immature xylem cells of the vascular bundles in the strawberry receptacle. To examine the importance of oxidative stress in ripening, gene expression was compared between fruit treated on-vine with a free radical generator and non-treated fruit. Of 46 genes induced, 20 were also ripening regulated. This might suggest that active gene expression is induced to cope with oxidative stress conditions during ripening or that the strawberry ripening transcriptional program is an oxidative stress-induced process. To gain insight into the hormonal control of non-climacteric fruit ripening, an additional microarray experiment was conducted comparing gene expression in fruit treated exogenously with auxin and control fruit. Novel auxin-dependent genes and processes were identified in addition to transcriptional programs acting independent of auxin mainly related to cell wall metabolism and stress response.
This article has been cited by other articles:
|
|
|
|
 
S. Conn, C. Curtin, A. Bezier, C. Franco, and W. Zhang
Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins
J. Exp. Bot.,
October 1, 2008;
59(13):
3621 - 3634.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Fait, K. Hanhineva, R. Beleggia, N. Dai, I. Rogachev, V. J. Nikiforova, A. R. Fernie, and A. Aharoni
Reconfiguration of the Achene and Receptacle Metabolic Networks during Strawberry Fruit Development
Plant Physiology,
October 1, 2008;
148(2):
730 - 750.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Griesser, F. Vitzthum, B. Fink, M. L. Bellido, C. Raasch, J. Munoz-Blanco, and W. Schwab
Multi-substrate flavonol O-glucosyltransferases from strawberry (Fragariaxananassa) achene and receptacle
J. Exp. Bot.,
July 1, 2008;
59(10):
2611 - 2625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Shulaev, S. S. Korban, B. Sosinski, A. G. Abbott, H. S. Aldwinckle, K. M. Folta, A. Iezzoni, D. Main, P. Arus, A. M. Dandekar, et al.
Multiple Models for Rosaceae Genomics
Plant Physiology,
July 1, 2008;
147(3):
985 - 1003.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Raab, J. A. Lopez-Raez, D. Klein, J. L. Caballero, E. Moyano, W. Schwab, and J. Munoz-Blanco
FaQR, Required for the Biosynthesis of the Strawberry Flavor Compound 4-Hydroxy-2,5-Dimethyl-3(2H)-Furanone, Encodes an Enone Oxidoreductase
PLANT CELL,
April 1, 2006;
18(4):
1023 - 1037.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Lunkenbein, M. Bellido, A. Aharoni, E. M.J. Salentijn, R. Kaldenhoff, H. A. Coiner, J. Munoz-Blanco, and W. Schwab
Cinnamate Metabolism in Ripening Fruit. Characterization of a UDP-Glucose:Cinnamate Glucosyltransferase from Strawberry
Plant Physiology,
March 1, 2006;
140(3):
1047 - 1058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Trainotti, A. Pavanello, and G. Casadoro
Different ethylene receptors show an increased expression during the ripening of strawberries: does such an increment imply a role for ethylene in the ripening of these non-climacteric fruits?
J. Exp. Bot.,
August 1, 2005;
56(418):
2037 - 2046.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Agius, I. Amaya, M. A. Botella, and V. Valpuesta
Functional analysis of homologous and heterologous promoters in strawberry fruits using transient expression
J. Exp. Bot.,
January 1, 2005;
56(409):
37 - 46.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Moyle, D. J. Fairbairn, J. Ripi, M. Crowe, and J. R. Botella
Developing pineapple fruit has a small transcriptome dominated by metallothionein
J. Exp. Bot.,
January 1, 2005;
56(409):
101 - 112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Castillejo, J. I. de la Fuente, P. Iannetta, M. A. Botella, and V. Valpuesta
Pectin esterase gene family in strawberry fruit: study of FaPE1, a ripening-specific isoform
J. Exp. Bot.,
April 1, 2004;
55(398):
909 - 918.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Thibaud-Nissen, R. T. Shealy, A. Khanna, and L. O. Vodkin
Clustering of Microarray Data Reveals Transcript Patterns Associated with Somatic Embryogenesis in Soybean
Plant Physiology,
May 1, 2003;
132(1):
118 - 136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
