Plant Physiology Preview
Published on July 18, 2002; 10.1104/pp.003780
Received February 13, 2002
Returned for revision March 21, 2002
Accepted April 11, 2002
Loss-of-Function Mutations in the Ethylene Receptor ETR1 Cause Enhanced Sensitivity and Exaggerated Response to Ethylene in Arabidopsis
Jesse D. Cancel and Paul B. Larsen *
Department of Biochemistry, University of California, Riverside, California 92521
* Corresponding author; email: paul.larsen{at}ucr.edu.
Ethylene signaling in Arabidopsis begins at a family of five ethylene receptors that regulate activity of a downstream mitogen-activated protein kinase kinase kinase, CTR1. Triple and quadruple loss-of-function ethylene receptor mutants display a constitutive ethylene response phenotype, indicating they function as negative regulators in this pathway. No ethylene-related phenotype has been described for single loss-of-function receptor mutants, although it was reported that etr1 loss-of-function mutants display a growth defect limiting plant size. In actuality, this apparent growth defect results from enhanced responsiveness to ethylene; a phenotype manifested in all tissues tested. The phenotype displayed by etr1 loss-of-function mutants was rescued by treatment with an inhibitor of ethylene perception, indicating that it is ethylene dependent. Identification of an ethylene-dependent phenotype for a loss-of-function receptor mutant gave a unique opportunity for genetic and biochemical analysis of upstream events in ethylene signaling, including demonstration that the dominant ethylene-insensitive phenotype of etr2-1 is partially dependent on ETR1. This work demonstrates that mutational loss of the ethylene receptor ETR1 alters responsiveness to ethylene in Arabidopsis and that enhanced ethylene response in Arabidopsis not only results in increased sensitivity but exaggeration of response.
This article has been cited by other articles:
|
|
|
|
 
J. M. Plett, J. Mathur, and S. Regan
Ethylene receptor ETR2 controls trichome branching by regulating microtubule assembly in Arabidopsis thaliana
J. Exp. Bot.,
September 1, 2009;
60(13):
3923 - 3933.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Gao, C.-K. Wen, B. M. Binder, Y.-F. Chen, J. Chang, Y.-H. Chiang, R. J. Kerris III, C. Chang, and G. E. Schaller
Heteromeric Interactions among Ethylene Receptors Mediate Signaling in Arabidopsis
J. Biol. Chem.,
August 29, 2008;
283(35):
23801 - 23810.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-r. Yin, K.-s. Chen, A. C. Allan, R.-m. Wu, B. Zhang, N. Lallu, and I. B. Ferguson
Ethylene-induced modulation of genes associated with the ethylene signalling pathway in ripening kiwifruit
J. Exp. Bot.,
May 1, 2008;
59(8):
2097 - 2108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Suzuki, S. Bajad, J. Shuman, V. Shulaev, and R. Mittler
The Transcriptional Co-activator MBF1c Is a Key Regulator of Thermotolerance in Arabidopsis thaliana
J. Biol. Chem.,
April 4, 2008;
283(14):
9269 - 9275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Grefen, K. Stadele, K. Ruzicka, P. Obrdlik, K. Harter, and J. Horak
Subcellular Localization and In Vivo Interactions of the Arabidopsis thaliana Ethylene Receptor Family Members
Mol Plant,
March 1, 2008;
1(2):
308 - 320.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zhong, Z. Lin, and D. Grierson
Tomato ethylene receptor-CTR interactions: visualization of NEVER-RIPE interactions with multiple CTRs at the endoplasmic reticulum
J. Exp. Bot.,
March 1, 2008;
59(4):
965 - 972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-F. Chen, S. N. Shakeel, J. Bowers, X.-C. Zhao, N. Etheridge, and G. E. Schaller
Ligand-induced Degradation of the Ethylene Receptor ETR2 through a Proteasome-dependent Pathway in Arabidopsis
J. Biol. Chem.,
August 24, 2007;
282(34):
24752 - 24758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Robles, J. S. Wampole, M. J. Christians, and P. B. Larsen
Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response, including ERF1 induction
J. Exp. Bot.,
July 1, 2007;
58(10):
2627 - 2639.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Christians and P. B. Larsen
Mutational loss of the prohibitin AtPHB3 results in an extreme constitutive ethylene response phenotype coupled with partial loss of ethylene-inducible gene expression in Arabidopsis seedlings
J. Exp. Bot.,
June 1, 2007;
58(8):
2237 - 2248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-H. Cho and S.-D. Yoo
ETHYLENE RESPONSE 1 Histidine Kinase Activity of Arabidopsis Promotes Plant Growth
Plant Physiology,
February 1, 2007;
143(2):
612 - 616.
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-H. Cao, J. Liu, X.-J. He, R.-L. Mu, H.-L. Zhou, S.-Y. Chen, and J.-S. Zhang
Modulation of Ethylene Responses Affects Plant Salt-Stress Responses
Plant Physiology,
February 1, 2007;
143(2):
707 - 719.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. M. Binder, R. C. O'Malley, W. Wang, T. C. Zutz, and A. B. Bleecker
Ethylene Stimulates Nutations That Are Dependent on the ETR1 Receptor
Plant Physiology,
December 1, 2006;
142(4):
1690 - 1700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Xie, Q. Liu, and C.-K. Wen
Receptor Signal Output Mediated by the ETR1 N Terminus Is Primarily Subfamily I Receptor Dependent
Plant Physiology,
October 1, 2006;
142(2):
492 - 508.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Chow and P. McCourt
Plant hormone receptors: perception is everything
Genes & Dev.,
August 1, 2006;
20(15):
1998 - 2008.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Resnick, C.-K. Wen, J. A. Shockey, and C. Chang
From The Cover: REVERSION-TO-ETHYLENE SENSITIVITY1, a conserved gene that regulates ethylene receptor function in Arabidopsis
PNAS,
May 16, 2006;
103(20):
7917 - 7922.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-C. Jing, J. H. M. Schippers, J. Hille, and P. P. Dijkwel
Ethylene-induced leaf senescence depends on age-related changes and OLD genes in Arabidopsis
J. Exp. Bot.,
November 1, 2005;
56(421):
2915 - 2923.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. De Paepe, L. De Grauwe, S. Bertrand, J. Smalle, and D. Van Der Straeten
The Arabidopsis mutant eer2 has enhanced ethylene responses in the light
J. Exp. Bot.,
September 1, 2005;
56(419):
2409 - 2420.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Trainotti, A. Pavanello, and G. Casadoro
Different ethylene receptors show an increased expression during the ripening of strawberries: does such an increment imply a role for ethylene in the ripening of these non-climacteric fruits?
J. Exp. Bot.,
August 1, 2005;
56(418):
2037 - 2046.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-F. CHEN, N. ETHERIDGE, and G. E. SCHALLER
Ethylene Signal Transduction
Ann. Bot.,
May 1, 2005;
95(6):
901 - 915.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Qu and G. E. Schaller
Requirement of the Histidine Kinase Domain for Signal Transduction by the Ethylene Receptor ETR1
Plant Physiology,
October 1, 2004;
136(2):
2961 - 2970.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. J. Klee
Ethylene Signal Transduction. Moving beyond Arabidopsis
Plant Physiology,
June 1, 2004;
135(2):
660 - 667.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Gao, Y.-F. Chen, M. D. Randlett, X.-C. Zhao, J. L. Findell, J. J. Kieber, and G. E. Schaller
Localization of the Raf-like Kinase CTR1 to the Endoplasmic Reticulum of Arabidopsis through Participation in Ethylene Receptor Signaling Complexes
J. Biol. Chem.,
September 5, 2003;
278(36):
34725 - 34732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Hall and A. B. Bleecker
Analysis of Combinatorial Loss-of-Function Mutants in the Arabidopsis Ethylene Receptors Reveals That the ers1 etr1 Double Mutant Has Severe Developmental Defects That Are EIN2 Dependent
PLANT CELL,
September 1, 2003;
15(9):
2032 - 2041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wang, A. E. Hall, R. O'Malley, and A. B. Bleecker
Canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor from Arabidopsis is not required for signal transmission
PNAS,
January 7, 2003;
100(1):
352 - 357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
