Plant Physiology Preview
Published on September 6, 2002; 10.1104/pp.005637
Received March 14, 2002
Returned for revision June 10, 2002
Accepted June 19, 2002
Soluble Invertase Expression Is an Early Target of Drought Stress during the Critical, Abortion-Sensitive Phase of Young Ovary Development in Maize
Mathias Neumann Andersen *, Folkard Asch , Yong Wu , Christian Richardt Jensen , Henrik Næsted , Vagn Overgaard Mogensen , and Karen Elaine Koch
Department of Crop Physiology and Soil Science, Danish Institute of Agricultural Sciences, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark (M.N.A.); Agricultural Chemistry Institute, Rheinische Friedrich-Wilhelms-Universität Bonn, Karlrobert-Kreiten-Strasse 13, D-53115 Bonn, Germany (F.A.); Horticultural Sciences Department, University of Florida, 1143 Fifield Hall, P.O. Box 110690, Gainesville, Florida 32611-6479 (Y.W., K.E.K.); Department of Agricultural Sciences, The Royal Veterinary and Agricultural University, Agrovej 10, DK-2630 Taastrup, Denmark (C.R.J., V.O.M.); and Department of Plant Physiology, University of Copenhagen,  ster Farimagsgade 2A, DK-1353 Copenhagen K, Denmark (H.N.)
* Corresponding author; email: mathiasn.andersen{at}agrsci.dk.
To distinguish their roles in early kernel development and stress, expression of soluble (Ivr2) and insoluble (Incw2) acid invertases was analyzed in young ovaries of maize (Zea mays) from 6 d before (-6 d) to 7 d after pollination (+7 d) and in response to perturbation by drought stress treatments. The Ivr2 soluble invertase mRNA was more abundant than the Incw2 mRNA throughout pre- and early post-pollination development (peaking at +3 d). In contrast, Incw2 mRNAs increased only after pollination. Drought repression of the Ivr2 soluble invertase also preceded changes in Incw2, with soluble activity responding before pollination (-4 d). Distinct profiles of Ivr2 and Incw2 mRNAs correlated with respective enzyme activities and indicated separate roles for these invertases during ovary development and stress. In addition, the drought-induced decrease and developmental changes of ovary hexose to sucrose ratio correlated with activity of soluble but not insoluble invertase. Ovary abscisic acid levels were increased by severe drought only at -6 d and did not appear to directly affect Ivr2 expression. In situ analysis showed localized activity and Ivr2 mRNA for soluble invertase at sites of phloem-unloading and expanding maternal tissues (greatest in terminal vascular zones and nearby cells of pericarp, pedicel, and basal nucellus). This early pattern of maternal invertase localization is clearly distinct from the well-characterized association of insoluble invertase with the basal endosperm later in development. This localization, the shifts in endogenous hexose to sucrose environment, and the distinct timing of soluble and insoluble invertase expression during development and stress collectively indicate a key role and critical sensitivity of the Ivr2 soluble invertase gene during the early, abortion-susceptible phase of development.
This article has been cited by other articles:
|
|
|
|
 
Y. Jin, D.-A. Ni, and Y.-L. Ruan
Posttranslational Elevation of Cell Wall Invertase Activity by Silencing Its Inhibitor in Tomato Delays Leaf Senescence and Increases Seed Weight and Fruit Hexose Level
PLANT CELL,
July 1, 2009;
21(7):
2072 - 2089.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. I. Zanor, S. Osorio, A. Nunes-Nesi, F. Carrari, M. Lohse, B. Usadel, C. Kuhn, W. Bleiss, P. Giavalisco, L. Willmitzer, et al.
RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and Fertility
Plant Physiology,
July 1, 2009;
150(3):
1204 - 1218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Werner, K. Holst, Y. Pors, A. Guivarc'h, A. Mustroph, D. Chriqui, B. Grimm, and T. Schmulling
Cytokinin deficiency causes distinct changes of sink and source parameters in tobacco shoots and roots
J. Exp. Bot.,
July 1, 2008;
59(10):
2659 - 2672.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Hirel, J. Le Gouis, B. Ney, and A. Gallais
The challenge of improving nitrogen use efficiency in crop plants: towards a more central role for genetic variability and quantitative genetics within integrated approaches
J. Exp. Bot.,
July 1, 2007;
58(9):
2369 - 2387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Boyer and J. E. McLaughlin
Functional reversion to identify controlling genes in multigenic responses: analysis of floral abortion
J. Exp. Bot.,
January 1, 2007;
58(2):
267 - 277.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Radchuk, L. Borisjuk, R. Radchuk, H.-H. Steinbiss, H. Rolletschek, S. Broeders, and U. Wobus
Jekyll Encodes a Novel Protein Involved in the Sexual Reproduction of Barley
PLANT CELL,
July 1, 2006;
18(7):
1652 - 1666.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. MAKELA, J. E. McLAUGHLIN, and J. S. BOYER
Imaging and Quantifying Carbohydrate Transport to the Developing Ovaries of Maize
Ann. Bot.,
October 1, 2005;
96(5):
939 - 949.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ishimaru, T. Hirose, T. Matsuda, A. Goto, K. Takahashi, H. Sasaki, T. Terao, R.-i. Ishii, R. Ohsugi, and T. Yamagishi
Expression Patterns of Genes Encoding Carbohydrate-metabolizing Enzymes and their Relationship to Grain Filling in Rice (Oryza sativa L.): Comparison of Caryopses Located at Different Positions in a Panicle
Plant Cell Physiol.,
April 1, 2005;
46(4):
620 - 628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Seebauer, S. P. Moose, B. J. Fabbri, L. D. Crossland, and F. E. Below
Amino Acid Metabolism in Maize Earshoots. Implications for Assimilate Preconditioning and Nitrogen Signaling
Plant Physiology,
December 1, 2004;
136(4):
4326 - 4334.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. McLAUGHLIN and J. S. BOYER
Sugar-responsive Gene Expression, Invertase Activity, and Senescence in Aborting Maize Ovaries at Low Water Potentials
Ann. Bot.,
November 1, 2004;
94(5):
675 - 689.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Boyer and M. E. Westgate
Grain yields with limited water
J. Exp. Bot.,
November 1, 2004;
55(407):
2385 - 2394.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Sun, K. Hunt, and B. A. Hauser
Ovule Abortion in Arabidopsis Triggered by Stress
Plant Physiology,
August 1, 2004;
135(4):
2358 - 2367.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. MCLAUGHLIN and J. S. BOYER
Glucose Localization in Maize Ovaries When Kernel Number Decreases at Low Water Potential and Sucrose is Fed to the Stems
Ann. Bot.,
July 1, 2004;
94(1):
75 - 86.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. J. Bate, X. Niu, Y. Wang, K. S. Reimann, and T. G. Helentjaris
An Invertase Inhibitor from Maize Localizes to the Embryo Surrounding Region during Early Kernel Development
Plant Physiology,
January 1, 2004;
134(1):
246 - 254.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
