Plant Physiol. PAM Fluorometers & Gas Exchange
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 3, 2002; 10.1104/pp.006262

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
130/2/796    most recent
pp.006262v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kao, Y.-Y.
Right arrow Articles by Tsai, C.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kao, Y.-Y.
Right arrow Articles by Tsai, C.-J.
Agricola
Right arrow Articles by Kao, Y.-Y.
Right arrow Articles by Tsai, C.-J.

Received March 25, 2002
Returned for revision May 10, 2002
Accepted June 23, 2002

Differential Expression of Two Distinct Phenylalanine Ammonia-Lyase Genes in Condensed Tannin-Accumulating and Lignifying Cells of Quaking Aspen

Yu-Ying Kao , Scott A. Harding , and Chung-Jui Tsai *

Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, Houghton, Michigan 49931

* Corresponding author; email: chtsai{at}mtu.edu.

Lignins, along with condensed tannins (CTs) and salicylate-derived phenolic glycosides, constitute potentially large phenylpropanoid carbon sinks in tissues of quaking aspen (Populus tremuloides Michx.). Metabolic commitment to each of these sinks varies during development and adaptation, and depends on L-phenylalanine ammonia-lyase (PAL), an enzyme catalyzing the deamination of L-phenylalanine to initiate phenylpropanoid metabolism. In Populus spp., PAL is encoded by multiple genes whose expression has been associated with lignification in primary and secondary tissues. We now report cloning two differentially expressed PAL cDNAs that exhibit distinct spatial associations with CT and lignin biosynthesis in developing shoot and root tissues of aspen. PtPAL1 was expressed in certain CT-accumulating, non-lignifying cells of stems, leaves, and roots, and the pattern of PtPAL1 expression varied coordinately with that of CT accumulation along the primary to secondary growth transition in stems. PtPAL2 was expressed in heavily lignified structural cells of shoots, but was also expressed in non-lignifying cells of root tips. Evidence of a role for Pt4CL2, encoding 4-coumarate:coenzyme A ligase, in determining CT sink strength was gained from cellular co-expression analysis with PAL1 and CTs, and from experiments in which leaf wounding increased PAL1 and 4CL2 expression as well as the relative allocation of carbon to CT with respect to phenolic glycoside, the dominant phenolic sink in aspen leaves. Leaf wounding also increased PAL2 and lignin pathway gene expression, but to a smaller extent. The absence of PAL2 in most CT-accumulating cells provides in situ support for the idea that PAL isoforms function in specific metabolic milieus.




This article has been cited by other articles:


Home page
 Plant CellHome page
T. Yamada, F. Matsuda, K. Kasai, S. Fukuoka, K. Kitamura, Y. Tozawa, H. Miyagawa, and K. Wakasa
Mutation of a Rice Gene Encoding a Phenylalanine Biosynthetic Enzyme Results in Accumulation of Phenylalanine and Tryptophan
PLANT CELL, May 1, 2008; 20(5): 1316 - 1329.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Lu, Y. Zhou, L. Li, and V. L. Chiang
Distinct Roles of Cinnamate 4-hydroxylase Genes in Populus
Plant Cell Physiol., July 1, 2006; 47(7): 905 - 914.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Paolocci, T. Bovone, N. Tosti, S. Arcioni, and F. Damiani
Light and an exogenous transcription factor qualitatively and quantitatively affect the biosynthetic pathway of condensed tannins in Lotus corniculatus leaves
J. Exp. Bot., April 1, 2005; 56(414): 1093 - 1103.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Rohde, K. Morreel, J. Ralph, G. Goeminne, V. Hostyn, R. De Rycke, S. Kushnir, J. Van Doorsselaere, J.-P. Joseleau, M. Vuylsteke, et al.
Molecular Phenotyping of the pal1 and pal2 Mutants of Arabidopsis thaliana Reveals Far-Reaching Consequences on Phenylpropanoid, Amino Acid, and Carbohydrate Metabolism
PLANT CELL, October 1, 2004; 16(10): 2749 - 2771.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. L. Jeong, H. Jiang, H.-S. Chen, C.-J. Tsai, and S. A. Harding
Metabolic Profiling of the Sink-to-Source Transition in Developing Leaves of Quaking Aspen
Plant Physiology, October 1, 2004; 136(2): 3364 - 3375.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D.-K. Ro and C. J. Douglas
Reconstitution of the Entry Point of Plant Phenylpropanoid Metabolism in Yeast (Saccharomyces cerevisiae): IMPLICATIONS FOR CONTROL OF METABOLIC FLUX INTO THE PHENYLPROPANOID PATHWAY
J. Biol. Chem., January 23, 2004; 279(4): 2600 - 2607.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Li, Q. Qian, Y. Zhou, M. Yan, L. Sun, M. Zhang, Z. Fu, Y. Wang, B. Han, X. Pang, et al.
BRITTLE CULM1, Which Encodes a COBRA-Like Protein, Affects the Mechanical Properties of Rice Plants
PLANT CELL, September 1, 2003; 15(9): 2020 - 2031.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2002 by the American Society of Plant Biologists