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Plant Physiology Preview Published on October 15, 2002; 10.1104/pp.009639
Received June 11, 2002 Phosphorus Effects on Metabolic Processes in Monoxenic Arbuscular Mycorrhiza Cultures
Department of Microbial Ecology, Ecology Building, Lund University, SE-223 62 Lund, Sweden (P.A.O., I.M.v.A., H.R.); School of Biological Sciences, The University of Sydney, New South Wales 2006, Australia (W.G.A.); and School of Biological Sciences, The University of New South Wales, Sydney 2052, Australia (A.E.A.) * Corresponding author; email: Pal_Axel.Olsson{at}mbioekol.lu.se.
The influence of external phosphorus (P) on carbon (C) allocation and metabolism as well as processes related to P metabolism was studied in monoxenic arbuscular mycorrhiza cultures of carrot (Daucus carota). Fungal hyphae of Glomus intraradices proliferated from the solid minimal medium containing the colonized roots into C-free liquid minimal medium with different P treatments. The fungus formed around three times higher biomass in P-free liquid medium than in medium with 2.5 mM inorganic P (high-P). Mycelium in the second experiment was harvested at an earlier growth stage to study metabolic processes when the mycelium was actively growing. P treatment influenced the root P content and [13C]glucose administered to the roots 7 d before harvest gave a negative correlation between root P content and 13C enrichment in arbuscular mycorrhiza fungal storage lipids in the extraradical hyphae. Eighteen percent of the enriched 13C in extraradical hyphae was recovered in the fatty acid 16:1
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5 from neutral lipids. Polyphosphate accumulated in hyphae even in P-free medium. No influence of P treatment on fungal acid phosphatase activity was observed, whereas the proportion of alkaline-phosphatase-active hyphae was highest in high-P medium. We demonstrated the presence of a motile tubular vacuolar system in G. intraradices. This system was rarely seen in hyphae subjected to the highest P treatment. We concluded that the direct responses of the extraradical hyphae to the P concentration in the medium are limited. The effects found in hyphae seemed instead to be related to increased availability of P to the host root.
