Plant Physiology Preview
Published on September 6, 2002; 10.1104/pp.009688
Received June 9, 2002
Returned for revision June 9, 2002
Accepted June 14, 2002
Transcription Profiling of the Early Gravitropic Response in Arabidopsis Using High-Density Oligonucleotide Probe Microarrays
Nick Moseyko , Tong Zhu , Hur-Song Chang , Xun Wang , and Lewis J. Feldman *
Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, California 94720-3102 (N.M., L.J.F.); and Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, California 92121 (T.Z., H.-S.C., X.W.)
* Corresponding author; email: feldman{at}nature.berkeley.edu.
Studies of plant tropisms, the directed growth toward or away from external stimuli such as light and gravity, began more than a century ago. Yet biochemical, physiological, and especially molecular mechanisms of plant tropic responses remain for the most part unclear. We examined expression of 8,300 genes during early stages of the gravitropic response using high-density oligonucleotide probe microarrays. Approximately 1.7% of the genes represented on the array exhibited significant expression changes within the first 30 min of gravity stimulation. Among gravity-induced genes were a number of genes previously implicated to be involved in gravitropism. However, a much larger number of the identified genes have not been previously associated with gravitropism. Because reorientation of plants may also expose plants to mechanical perturbations, we also compared the effects of a gentle mechanical perturbation on mRNA levels during the gravity response. It was found that approximately 39% of apparently gravity-regulated genes were also regulated by the mechanical perturbation caused by plant reorientation. Our study revealed the induction of complex gene expression patterns as a consequence of gravitropic reorientation and points to an interplay between the gravitropic and mechanical responses and to the extreme sensitivity of plants to even very gentle mechanical perturbations.
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