Plant Physiology Preview
Published on January 9, 2003; 10.1104/pp.009985
Received June 12, 2002
Returned for revision July 31, 2002
Accepted November 3, 2002
Genomic and Proteomic Analysis of Mitochondrial Carrier Proteins in Arabidopsis
A. Harvey Millar * and Joshua L. Heazlewood
Plant Molecular Biology Group, School of Biomedical and Chemical Sciences, The University of Western Australia, Crawley 6009, Western Australia, Australia
* Corresponding author; email: hmillar{at}cyllene.uwa.edu.au.
Plant mitochondria maintain metabolic communication with the cytosol through a family of carrier proteins. In Arabidopsis, a subset of 45 putative genes encoding members of this family have been identified based on generalized mitochondrial carrier features. No gene clusters are apparent and few of the predicted protein products have mitochondrial targeting sequences recognized by bioinformatic predictors. Only nine genes are currently represented by more than 10 expressed sequence tags at The Institute for Genomic Research. Analyses of public microarray experiments reveal differential expression profiles of the more highly expressed members of this gene family in different plant organs and in response to plant hormone application and environmental stresses. A comparison of this Arabidopsis carrier subset (45) to the yeast gene family (35) reveals 10 orthologous groups between the two species. Recent surveys of the Arabidopsis mitochondrial proteome by two-dimensional gel separations have not identified any of these carrier proteins, presumably because of their hydrophobicity and basicity. Isolating integral membrane proteins from Arabidopsis mitochondria, using one-dimensional electrophoresis for protein separation and tandem mass spectrometry-based sequencing of doubly charged peptides, we have unequivocally identified specific carrier gene products located in mitochondria. This approach has identified six of the nine carriers represented highly in expressed sequence tag databases: adenine nucleotide translocator (At3g8580 and At5g13490), dicarboxylate/tricarboxylate carrier (At5g19760), phosphate carrier (At5g14040), uncoupling protein (At3g54110), and a carrier gene of unknown function (At4g01100). Overall, the combined transcript and protein expression data indicates that only a small subset of the carrier family of genes provide the majority of carrier proteins of Arabidopsis mitochondria.
This article has been cited by other articles:
|
|
|
|
 
F. Palmieri, B. Rieder, A. Ventrella, E. Blanco, P. T. Do, A. Nunes-Nesi, A. U. Trauth, G. Fiermonte, J. Tjaden, G. Agrimi, et al.
Molecular Identification and Functional Characterization of Arabidopsis thaliana Mitochondrial and Chloroplastic NAD+ Carrier Proteins
J. Biol. Chem.,
November 6, 2009;
284(45):
31249 - 31259.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Palmieri, A. Santoro, F. Carrari, E. Blanco, A. Nunes-Nesi, R. Arrigoni, F. Genchi, A. R. Fernie, and F. Palmieri
Identification and Characterization of ADNT1, a Novel Mitochondrial Adenine Nucleotide Transporter from Arabidopsis
Plant Physiology,
December 1, 2008;
148(4):
1797 - 1808.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Eubel, E. H. Meyer, N. L. Taylor, J. D. Bussell, N. O'Toole, J. L. Heazlewood, I. Castleden, I. D. Small, S. M. Smith, and A. H. Millar
Novel Proteins, Putative Membrane Transporters, and an Integrated Metabolic Network Are Revealed by Quantitative Proteomic Analysis of Arabidopsis Cell Culture Peroxisomes
Plant Physiology,
December 1, 2008;
148(4):
1809 - 1829.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kirchberger, M. Leroch, M. A. Huynen, M. Wahl, H. E. Neuhaus, and J. Tjaden
Molecular and Biochemical Analysis of the Plastidic ADP-glucose Transporter (ZmBT1) from Zea mays
J. Biol. Chem.,
August 3, 2007;
282(31):
22481 - 22491.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Thuswaldner, J. O. Lagerstedt, M. Rojas-Stutz, K. Bouhidel, C. Der, N. Leborgne-Castel, A. Mishra, F. Marty, B. Schoefs, I. Adamska, et al.
Identification, Expression, and Functional Analyses of a Thylakoid ATP/ADP Carrier from Arabidopsis
J. Biol. Chem.,
March 23, 2007;
282(12):
8848 - 8859.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Komatsu, H. Konishi, and M. Hashimoto
The proteomics of plant cell membranes
J. Exp. Bot.,
January 1, 2007;
58(1):
103 - 112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Palmieri, R. Arrigoni, E. Blanco, F. Carrari, M. I. Zanor, C. Studart-Guimaraes, A. R. Fernie, and F. Palmieri
Molecular Identification of an Arabidopsis S-Adenosylmethionine Transporter. Analysis of Organ Distribution, Bacterial Expression, Reconstitution into Liposomes, and Functional Characterization
Plant Physiology,
November 1, 2006;
142(3):
855 - 865.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Rolland, M. Ferro, G. Ephritikhine, A. Marmagne, C. Ramus, S. Brugiere, D. Salvi, D. Seigneurin-Berny, J. Bourguignon, H. Barbier-Brygoo, et al.
A versatile method for deciphering plant membrane proteomes
J. Exp. Bot.,
April 1, 2006;
57(7):
1579 - 1589.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Borecky, F. T. S. Nogueira, K. A. P. de Oliveira, I. G. Maia, A. E. Vercesi, and P. Arruda
The plant energy-dissipating mitochondrial systems: depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicots
J. Exp. Bot.,
March 1, 2006;
57(4):
849 - 864.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Heazlewood, J. Tonti-Filippini, R. E. Verboom, and A. H. Millar
Combining Experimental and Predicted Datasets for Determination of the Subcellular Location of Proteins in Arabidopsis
Plant Physiology,
October 1, 2005;
139(2):
598 - 609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Leroch, S. Kirchberger, I. Haferkamp, M. Wahl, H. E. Neuhaus, and J. Tjaden
Identification and Characterization of a Novel Plastidic Adenine Nucleotide Uniporter from Solanum tuberosum
J. Biol. Chem.,
May 6, 2005;
280(18):
17992 - 18000.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Heazlewood and A. H. Millar
AMPDB: the Arabidopsis Mitochondrial Protein Database
Nucleic Acids Res.,
January 1, 2005;
33(suppl_1):
D605 - D610.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Alexandersson, G. Saalbach, C. Larsson, and P. Kjellbom
Arabidopsis Plasma Membrane Proteomics Identifies Components of Transport, Signal Transduction and Membrane Trafficking
Plant Cell Physiol.,
November 15, 2004;
45(11):
1543 - 1556.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. del Arco and J. Satrustegui
Identification of a Novel Human Subfamily of Mitochondrial Carriers with Calcium-binding Domains
J. Biol. Chem.,
June 4, 2004;
279(23):
24701 - 24713.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Heazlewood, J. S. Tonti-Filippini, A. M. Gout, D. A. Day, J. Whelan, and A. H. Millar
Experimental Analysis of the Arabidopsis Mitochondrial Proteome Highlights Signaling and Regulatory Components, Provides Assessment of Targeting Prediction Programs, and Indicates Plant-Specific Mitochondrial Proteins
PLANT CELL,
January 1, 2004;
16(1):
241 - 256.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Heazlewood, K. A. Howell, J. Whelan, and A. H. Millar
Towards an Analysis of the Rice Mitochondrial Proteome
Plant Physiology,
May 1, 2003;
132(1):
230 - 242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
