Plant Physiology Preview
Published on November 14, 2002; 10.1104/pp.010348
Received June 20, 2002
Returned for revision August 2, 2002
Accepted August 19, 2002
A Rice Mutant Defective in Si Uptake
Jian Feng Ma *, Kazunori Tamai , Masahiko Ichii , and Guo Feng Wu
Faculty of Agriculture, Kagawa University, Ikenobe 2393, Miki-cho, Kita-gun, Kagawa 761-0795, Japan
* Corresponding author; email: maj{at}ag.kagawa-u.ac.jp.
Rice (Oryza sativa) accumulates silicon (Si) in the tops to levels up to 10.0% of shoot dry weight, but the mechanism responsible for high Si uptake by rice roots is not understood. We isolated a rice mutant (GR1) that is defective in active Si uptake by screening M2 seeds (64,000) of rice cv Oochikara that were treated with 10-3 M sodium azide for 6 h at 25oC. There were no phenotypic differences between wild type (WT) and GR1 except that the leaf blade of GR1 remained droopy when Si was supplied. Uptake experiments showed that Si uptake by GR1 was significantly lower than that by WT at both low and high Si concentrations. However, there was no difference in the uptake of other nutrients such as phosphorus and potassium. Si concentration in the xylem sap of WT was 33-fold that of the external solution, but that of GR1 was 3-fold higher than the external solution at 0.15 mM Si. Si uptake by WT was inhibited by metabolic inhibitors including NaCN and 2,4-dinitrophenol and by low temperature, whereas Si uptake by GR1 was not inhibited by these agents. These results suggest that an active transport system for Si uptake is disrupted in GR1. Analysis of F2 populations between GR1 and WT showed that roots with high Si uptake and roots with low Si uptake segregated at a 3:1 ratio, suggesting that GR1 is a recessive mutant of Si uptake.
This article has been cited by other articles:
|
|
|
|
 
R.-Y. Li, Y. Ago, W.-J. Liu, N. Mitani, J. Feldmann, S. P. McGrath, J. F. Ma, and F.-J. Zhao
The Rice Aquaporin Lsi1 Mediates Uptake of Methylated Arsenic Species
Plant Physiology,
August 1, 2009;
150(4):
2071 - 2080.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Mitani, N. Yamaji, and J. F. Ma
Identification of Maize Silicon Influx Transporters
Plant Cell Physiol.,
January 1, 2009;
50(1):
5 - 12.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. Ma, N. Yamaji, N. Mitani, X.-Y. Xu, Y.-H. Su, S. P. McGrath, and F.-J. Zhao
Transporters of arsenite in rice and their role in arsenic accumulation in rice grain
PNAS,
July 22, 2008;
105(29):
9931 - 9935.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Yamaji, N. Mitatni, and J. F. Ma
A Transporter Regulating Silicon Distribution in Rice Shoots
PLANT CELL,
May 1, 2008;
20(5):
1381 - 1389.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. Ma, N. Yamaji, K. Tamai, and N. Mitani
Genotypic Difference in Silicon Uptake and Expression of Silicon Transporter Genes in Rice
Plant Physiology,
November 1, 2007;
145(3):
919 - 924.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Yamaji and J. F. Ma
Spatial Distribution and Temporal Variation of the Rice Silicon Transporter Lsi1
Plant Physiology,
March 1, 2007;
143(3):
1306 - 1313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Nikolic, N. Nikolic, Y. Liang, E. A. Kirkby, and V. Romheld
Germanium-68 as an Adequate Tracer for Silicon Transport in Plants. Characterization of Silicon Uptake in Different Crop Species
Plant Physiology,
January 1, 2007;
143(1):
495 - 503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. HODSON, P. J. WHITE, A. MEAD, and M. R. BROADLEY
Phylogenetic Variation in the Silicon Composition of Plants
Ann. Bot.,
November 1, 2005;
96(6):
1027 - 1046.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Mitani, J. F. Ma, and T. Iwashita
Identification of the Silicon Form in Xylem Sap of Rice (Oryza sativa L.)
Plant Cell Physiol.,
February 1, 2005;
46(2):
279 - 283.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. Ma, N. Mitani, S. Nagao, S. Konishi, K. Tamai, T. Iwashita, and M. Yano
Characterization of the Silicon Uptake System and Molecular Mapping of the Silicon Transporter Gene in Rice
Plant Physiology,
October 1, 2004;
136(2):
3284 - 3289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
