Plant Physiology Preview
Published on February 24, 2002; 10.1104/pp.010646
Received July 23, 2001
Returned for revision October 7, 2001
Accepted November 29, 2001
Copper Amine Oxidase Expression in Defense Responses to
Wounding and Ascochyta rabiei Invasion
Giuseppina Rea , Ouissal Metoui , Alessandro Infantino , Rodolfo Federico , and Riccardo Angelini *
Dipartimento di Biologia, Università degli Studi "Roma Tre," Viale Guglielmo Marconi 446, 00146 Rome, Italy; and Istituto Sperimentale per la Patologia Vegetale, Via Carlo Giuseppe Bertero 22, 00156 Rome, Italy
* Corresponding author; email: angelini{at}bio.uniroma3.it.
Wounding chickpea (Cicer arietinum) internodes or cotyledons resulted in an increase in the steady-state level of copper amine oxidase (CuAO) expression both locally and systemically. Dissection of the molecular mechanisms controlling CuAO expression indicated that jasmonic acid worked as a potent inducer of the basal and wound-inducible CuAO expression, whereas salicylic acid and abscisic acid caused a strong reduction of the wound-induced CuAO expression, without having any effect on the basal levels. Epicotyl treatment with the CuAO mechanism-based inhibitor 2-bromoethylamine decreased hydrogen peroxide (H2O2) levels in all the internodes, as evidenced in vivo by 3,3'-diaminobenzidine oxidation. Moreover, inhibitor pretreatment of wounded epicotyls resulted in a lower accumulation of H2O2 both at the wound site and in distal organs. In vivo CuAO inhibition by 2-bromoethylamine after inoculation of resistant chickpea cv "Sultano" with Ascochyta rabiei resulted in the development of extended necrotic lesions, with extensive cell damage occurring in sclerenchyma and cortical parenchyma tissues. These results, besides stressing the fine-tuning by key signaling molecules in wound-induced CuAO regulation, demonstrate that local and systemic CuAO induction is essential for H2O2 production in response to wounding and indicate the relevance of these enzymes in protection against pathogens.
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