Plant Physiology Preview
Published on April 9, 2002; 10.1104/pp.010869
Received September 24, 2001
Returned for revision December 12, 2001
Accepted February 16, 2002
Adaptation of H+-Pumping and Plasma Membrane
H+ ATPase Activity in Proteoid Roots of White Lupin under
Phosphate Deficiency
Feng Yan *, Yiyong Zhu , Caroline Müller , Christian Zörb , and Sven Schubert
Institute of Plant Nutrition, Interdisciplinary Research Center, Justus Liebig University, Heinrich-Buff-Ring 26--32, D--35392 Giessen, Germany
* Corresponding author; email: feng.yan{at}ernaehrung.unigiessen.de.
White lupin (Lupinus albus) is able to adapt to phosphorus deficiency by producing proteoid roots that release a huge amount of organic acids, resulting in mobilization of sparingly soluble soil phosphate in rhizosphere. The mechanisms responsible for the release of organic acids by proteoid root cells, especially the trans-membrane transport processes, have not been elucidated. Because of high cytosolic pH, the release of undissociated organic acids is not probable. In the present study, we focused on H+ export by plasma membrane H+ ATPase in active proteoid roots. In vivo, rhizosphere acidification of active proteoid roots was vanadate sensitive. Plasma membranes were isolated from proteoid roots and lateral roots from P-deficient and -sufficient plants. In vitro, in comparison with two types of lateral roots and proteoid roots of P-sufficient plants, the following increase of the various parameters was induced in active proteoid roots of P-deficient plants: (a) hydrolytic ATPase activity, (b) Vmax and Km, (c) H+ ATPase enzyme concentration of plasma membrane, (d) H+-pumping activity, (e) pH gradient across the membrane of plasmalemma vesicles, and (f) passive H+ permeability of plasma membrane. In addition, lower vanadate sensitivity and more acidic pH optimum were determined for plasma membrane ATPase of active proteoid roots. Our data support the hypothesis that in active proteoid root cells, H+ and organic anions are exported separately, and that modification of plasma membrane H+ ATPase is essential for enhanced rhizosphere acidification by active proteoid roots.
This article has been cited by other articles:
|
|
|
|
 
C. Chang, Y. Hu, S. Sun, Y. Zhu, G. Ma, and G. Xu
Proton pump OsA8 is linked to phosphorus uptake and translocation in rice
J. Exp. Bot.,
February 1, 2009;
60(2):
557 - 565.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Zhou, M. Yamagishi, M. Osaki, and K. Masuda
Sugar signalling mediates cluster root formation and phosphorus starvation-induced gene expression in white lupin
J. Exp. Bot.,
July 1, 2008;
59(10):
2749 - 2756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. V. Badri, V. M. Loyola-Vargas, C. D. Broeckling, C. De-la-Pena, M. Jasinski, D. Santelia, E. Martinoia, L. W. Sumner, L. M. Banta, F. Stermitz, et al.
Altered Profile of Secondary Metabolites in the Root Exudates of Arabidopsis ATP-Binding Cassette Transporter Mutants
Plant Physiology,
February 1, 2008;
146(2):
762 - 771.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Yang, J. F. You, Y. Y. Li, P. Wu, and S. J. Zheng
Magnesium Enhances Aluminum-Induced Citrate Secretion in Rice Bean Roots (Vigna umbellata) by Restoring Plasma Membrane H+-ATPase Activity
Plant Cell Physiol.,
January 1, 2007;
48(1):
66 - 73.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shen, J. Chen, Z. Wang, C. Yang, T. Sasaki, Y. Yamamoto, H. Matsumoto, and X. Yan
Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation
J. Exp. Bot.,
March 1, 2006;
57(6):
1353 - 1362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zhu, F. Yan, C. Zorb, and S. Schubert
A Link Between Citrate and Proton Release by Proteoid Roots of White Lupin (Lupinus albus L.) Grown Under Phosphorus-deficient Conditions?
Plant Cell Physiol.,
June 1, 2005;
46(6):
892 - 901.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shen, L. F. He, T. Sasaki, Y. Yamamoto, S. J. Zheng, A. Ligaba, X. L. Yan, S. J. Ahn, M. Yamaguchi, H. Sasakawa, et al.
Citrate Secretion Coupled with the Modulation of Soybean Root Tip under Aluminum Stress. Up-Regulation of Transcription, Translation, and Threonine-Oriented Phosphorylation of Plasma Membrane H+-ATPase
Plant Physiology,
May 1, 2005;
138(1):
287 - 296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-H. Zhang, P. R. Ryan, and S. D. Tyerman
Citrate-Permeable Channels in the Plasma Membrane of Cluster Roots from White Lupin
Plant Physiology,
November 1, 2004;
136(3):
3771 - 3783.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kihara, T. Wada, Y. Suzuki, T. Hara, and H. Koyama
Alteration of Citrate Metabolism in Cluster Roots of White Lupin
Plant Cell Physiol.,
September 15, 2003;
44(9):
901 - 908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ohno, H. Koyama, and T. Hara
Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion
Plant Cell Physiol.,
February 15, 2003;
44(2):
156 - 162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
