Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 17, 2002; 10.1104/pp.011353

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
130/3/1230    most recent
pp.011353v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Criqui, M. C.
Right arrow Articles by Genschik, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Criqui, M. C.
Right arrow Articles by Genschik, P.
Agricola
Right arrow Articles by Criqui, M. C.
Right arrow Articles by Genschik, P.

Received July 16, 2002
Accepted August 7, 2002

Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family

Marie Claire Criqui , Janice de Almeida Engler , Alain Camasses , Arnaud Capron , Yves Parmentier , Dirk Inzé , and Pascal Genschik *

Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (M.C.C., A.C., Y.P., P.G.); Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, B-9000 Gent, Belgium (J.d.A.E., D.I.); and Unité Propre de Recherche 9005 Mecanismes Moleculaires de la Division Cellulaire et du Developpement, 21 rue René Descartes, 67084, Strasbourg, France (A.C.)

* Corresponding author; email: Pascal.Genschik{at}ibmp-ulpu-strasbg.fr.

The anaphase promoting complex or cyclosome is the ubiquitin-ligase that targets destruction box-containing proteins for proteolysis during the cell cycle. Anaphase promoting complex or cyclosome and its activator (the fizzy and fizzy-related) proteins work together with ubiquitin-conjugating enzymes (UBCs) (E2s). One class of E2s (called E2-C) seems specifically involved in cyclin B1 degradation. Although it has recently been shown that mammalian E2-C is regulated at the protein level during the cell cycle, not much is known concerning the expression of these genes. Arabidopsis encodes two genes belonging to the E2-C gene family (called UBC19 and UBC20). We found that UBC19 is able to complement fission yeast (Schizosaccharomyces pombe) UbcP4-140 mutant, indicating that the plant protein can functionally replace its yeast ortholog for protein degradation during mitosis. In situ hybridization experiments were performed to study the expression of the E2-C genes in various tissues of plants. Their transcripts were always, but not exclusively, found in tissues active for cell division. Thus, the UBC19/20 E2s may have a key function during cell cycle, but may also be involved in ubiquitylation reactions occurring during differentiation and/or in differentiated cells. Finally, we showed that a translational fusion protein between UBC19 and green fluorescent protein localized both in the cytosol and the nucleus in stable transformed tobacco (Nicotiana tabacum cv Bright Yellow 2) cells.




This article has been cited by other articles:


Home page
 DevelopmentHome page
K. Marrocco, A. Thomann, Y. Parmentier, P. Genschik, and M. C. Criqui
The APC/C E3 ligase remains active in most post-mitotic Arabidopsis cells and is required for proper vasculature development and organization
Development, May 1, 2009; 136(9): 1475 - 1485.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Kato, I. Galis, S. Suzuki, S. Araki, T. Demura, M.-C. Criqui, T. Potuschak, P. Genschik, H. Fukuda, K. Matsuoka, et al.
Preferential Up-Regulation of G2/M Phase-Specific Genes by Overexpression of the Hyperactive Form of NtmybA2 Lacking Its Negative Regulation Domain in Tobacco BY-2 Cells
Plant Physiology, April 1, 2009; 149(4): 1945 - 1957.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Kraft, S. L. Stone, L. Ma, N. Su, Y. Gao, O.-S. Lau, X.-W. Deng, and J. Callis
Genome Analysis and Functional Characterization of the E2 and RING-Type E3 Ligase Ubiquitination Enzymes of Arabidopsis
Plant Physiology, December 1, 2005; 139(4): 1597 - 1611.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
C. J. Fong, L. D. Burgoon, and T. R. Zacharewski
Comparative Microarray Analysis of Basal Gene Expression in Mouse Hepa-1c1c7 Wild-Type and Mutant Cell Lines
Toxicol. Sci., August 1, 2005; 86(2): 342 - 353.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2002 by the American Society of Plant Biologists