Plant Physiology Preview
Published on January 9, 2003; 10.1104/pp.012047
Received August 1, 2002
Returned for revision September 22, 2002
Accepted October 19, 2002
Recently Duplicated Maize R2R3 Myb Genes Provide Evidence for Distinct Mechanisms of Evolutionary Divergence after Duplication
Anusha P. Dias , Edward L. Braun , Michael D. McMullen , and Erich Grotewold *
Department of Plant Biology and Plant Biotechnology Center, Ohio State University, Columbus, Ohio 43210 (A.P.D., E.G.); Department of Zoology, University of Florida, Gainesville, Florida 32611 (E.L.B.); and Plant Genetics Research and Plant Science Units, United States Department of Agriculture-Agricultural Research Service, University of Missouri, Columbia, Missouri 65211 (M.D.M.)
* Corresponding author; email: grotewold.1{at}osu.edu.
R2R3 Myb genes are widely distributed in the higher plants and comprise one of the largest known families of regulatory proteins. Here, we provide an evolutionary framework that helps explain the origin of the plant-specific R2R3 Myb genes from widely distributed R1R2R3 Myb genes, through a series of well-established steps. To understand the routes of sequence divergence that followed Myb gene duplication, we supplemented the information available on recently duplicated maize (Zea mays) R2R3 Myb genes (C1/Pl1 and P1/P2) by cloning and characterizing ZmMyb-IF35 and ZmMyb-IF25. These two genes correspond to the recently expanded P-to-A group of maize R2R3 Myb genes. Although the origins of C1/Pl1 and ZmMyb-IF35/ZmMyb-IF25 are associated with the segmental allotetraploid origin of the maize genome, other gene duplication events also shaped the P-to-A clade. Our analyses indicate that some recently duplicated Myb gene pairs display substantial differences in the numbers of synonymous substitutions that have accumulated in the conserved MYB domain and the divergent C-terminal regions. Thus, differences in the accumulation of substitutions during evolution can explain in part the rapid divergence of C-terminal regions for these proteins in some cases. Contrary to previous studies, we show that the divergent C termini of these R2R3 MYB proteins are subject to purifying selection. Our results provide an in-depth analysis of the sequence divergence for some recently duplicated R2R3 Myb genes, yielding important information on general patterns of evolution for this large family of plant regulatory genes.
This article has been cited by other articles:
|
|
|
|
 
M. Chen, Z. Xu, L. Xia, L. Li, X. Cheng, J. Dong, Q. Wang, and Y. Ma
Cold-induced modulation and functional analyses of the DRE-binding transcription factor gene, GmDREB3, in soybean (Glycine max L.)
J. Exp. Bot.,
January 1, 2009;
60(1):
121 - 135.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Yilmaz, M. Y. Nishiyama Jr., B. G. Fuentes, G. M. Souza, D. Janies, J. Gray, and E. Grotewold
GRASSIUS: A Platform for Comparative Regulatory Genomics across the Grasses
Plant Physiology,
January 1, 2009;
149(1):
171 - 180.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Alandete-Saez, M. Ron, and S. McCormick
GEX3, Expressed in the Male Gametophyte and in the Egg Cell of Arabidopsis thaliana, Is Essential for Micropylar Pollen Tube Guidance and Plays a Role during Early Embryogenesis
Mol Plant,
July 1, 2008;
1(4):
586 - 598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Punwani, D. S. Rabiger, and G. N. Drews
MYB98 Positively Regulates a Battery of Synergid-Expressed Genes Encoding Filiform Apparatus Localized Proteins
PLANT CELL,
August 1, 2007;
19(8):
2557 - 2568.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. B. Lai, J. A. Nadeau, J. Lucas, E.-K. Lee, T. Nakagawa, L. Zhao, M. Geisler, and F. D. Sack
The Arabidopsis R2R3 MYB Proteins FOUR LIPS and MYB88 Restrict Divisions Late in the Stomatal Cell Lineage
PLANT CELL,
October 1, 2005;
17(10):
2754 - 2767.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Davidson, R. Tirouvanziam, L. A. Herzenberg, and J. S. Lipsick
Functional Evolution of the Vertebrate Myb Gene Family: B-Myb, but Neither A-Myb nor c-Myb, Complements Drosophila Myb in Hemocytes
Genetics,
January 1, 2005;
169(1):
215 - 229.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. F. Heine, J. M. Hernandez, and E. Grotewold
Two Cysteines in Plant R2R3 MYB Domains Participate in REDOX-dependent DNA Binding
J. Biol. Chem.,
September 3, 2004;
279(36):
37878 - 37885.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Elomaa, A. Uimari, M. Mehto, V. A. Albert, R. A.E. Laitinen, and T. H. Teeri
Activation of Anthocyanin Biosynthesis in Gerbera hybrida (Asteraceae) Suggests Conserved Protein-Protein and Protein-Promoter Interactions between the Anciently Diverged Monocots and Eudicots
Plant Physiology,
December 1, 2003;
133(4):
1831 - 1842.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. O. Marian, S. J. Bordoli, M. Goltz, R. A. Santarella, L. P. Jackson, O. Danilevskaya, M. Beckstette, R. Meeley, and H. W. Bass
The Maize Single myb histone 1 Gene, Smh1, Belongs to a Novel Gene Family and Encodes a Protein That Binds Telomere DNA Repeats in Vitro
Plant Physiology,
November 1, 2003;
133(3):
1336 - 1350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
