Plant Physiology Preview
Published on January 9, 2003; 10.1104/pp.014555
Received September 13, 2002
Returned for revision October 25, 2002
Accepted November 14, 2002
AtCSLA7, a Cellulose Synthase-Like Putative Glycosyltransferase, Is Important for Pollen Tube Growth and Embryogenesis in Arabidopsis
Florence Goubet , Audrey Misrahi , Soon Ki Park , Zhinong Zhang , David Twell , and Paul Dupree *
Department of Biochemistry, University of Cambridge, Building O, Downing Site, Cambridge CB2 1QW, United Kingdom (F.G., A.M., Z.Z., P.D.); and Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom (S.K.P., D.T.)
* Corresponding author; email: p.dupree{at}bioc.cam.ac.uk.
The cellulose synthase-like proteins are a large family of proteins in plants thought to be processive polysaccharide  -glycosyltransferases. We have characterized an Arabidopsis mutant with a transposon insertion in the gene encoding AtCSLA7 of the CSLA subfamily. Analysis of the transmission efficiency of the insertion indicated that AtCSLA7 is important for pollen tube growth. Moreover, the homozygous insertion was embryo lethal. A detailed analysis of seed developmental progression revealed that mutant embryos developed more slowly than wild-type siblings. The mutant embryos also showed abnormal cell patterning and they arrested at a globular stage. The defective embryonic development was associated with reduced proliferation and failed cellularization of the endosperm. AtCSLA7 is widely expressed, and is likely to be required for synthesis of a cell wall polysaccharide found throughout the plant. Our results suggest that this polysaccharide is essential for cell wall structure or for signaling during plant embryo development.
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