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Published on February 27, 2003; 10.1104/pp.102.007765

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Received May 7, 2002
Returned for revision July 31, 2002
Accepted November 26, 2002

Carbon Export from Arbuscular Mycorrhizal Roots Involves the Translocation of Carbohydrate as well as Lipid

Berta Bago , Philip E. Pfeffer , Jehad Abubaker , Jeongwon Jun , James W. Allen , Janine Brouillette , David D. Douds , Peter J. Lammers , and Yair Shachar-Hill *

Eastern Regional Research Center (U.S. Department of Agriculture/Agricultural Research Service), Wyndmoor, Pennsylvania 19038 (B.B., P.E.P., J.B., D.D.D.); Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003 (J.A., J.J., J.W.A., P.J.L., Y.S.-H.); and Plant Biology Department, Michigan State University, East Lansing, Michigan 48823 (J.W.A., Y.S.-H.)

* Corresponding author; email: yairhill{at}msu.edu.

Arbuscular mycorrhizal (AM) fungi take up photosynthetically fixed carbon from plant roots and translocate it to their external mycelium. Previous experiments have shown that fungal lipid synthesized from carbohydrate in the root is one form of exported carbon. In this study, an analysis of the labeling in storage and structural carbohydrates after 13C1 glucose was provided to AM roots shows that this is not the only pathway for the flow of carbon from the intraradical to the extraradical mycelium (ERM). Labeling patterns in glycogen, chitin, and trehalose during the development of the symbiosis are consistent with a significant flux of exported glycogen. The identification, among expressed genes, of putative sequences for glycogen synthase, glycogen branching enzyme, chitin synthase, and for the first enzyme in chitin synthesis (glutamine fructose-6-phosphate aminotransferase) is reported. The results of quantifying glycogen synthase gene expression within mycorrhizal roots, germinating spores, and ERM are consistent with labeling observations using 13C-labeled acetate and glycerol, both of which indicate that glycogen is synthesized by the fungus in germinating spores and during symbiosis. Implications of the labeling analyses and gene sequences for the regulation of carbohydrate metabolism are discussed, and a 4-fold role for glycogen in the AM symbiosis is proposed: sequestration of hexose taken from the host, long-term storage in spores, translocation from intraradical mycelium to ERM, and buffering of intracellular hexose levels throughout the life cycle.




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