Plant Physiology Preview
Published on January 23, 2003; 10.1104/pp.102.011007
Received July 8, 2002
Returned for revision August 7, 2002
Accepted October 16, 2002
Osmotic Stress Tolerance of Transgenic Tobacco Expressing a Gene Encoding a Membrane-Located Receptor-Like Protein from Tobacco Plants
Takashi Tamura , Kojiro Hara , Yube Yamaguchi , Nozomu Koizumi , and Hiroshi Sano *
Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630-0192, Japan
* Corresponding author; email: sano{at}gtc.aist-nara.ac.jp.
Tobacco (Nicotiana tabacum) genes regulated during the early stage of responses to wounding were screened by a modified fluorescence differential display method. Among 28 genes initially identified, a particular clone designated NtC7 was subjected to further analysis. Its transcripts were found to accumulate rapidly and transiently within 1 h upon treatments with not only wounding but also salt and osmotic stresses. However, jasmonic and abscisic acids and ethylene did not effectively induce NtC7 transcripts. Amino acid sequence analysis suggested NtC7 to be a new type of transmembrane protein that belongs to the receptor-like protein family, and a membrane location was confirmed in onion (Allium cepa) epidermis cells transiently expressing an NtC7-green fluorescent protein fusion protein. Seeds of transgenic tobacco overexpressing NtC7 normally germinated and grew in the presence of 500 mM mannitol, but not in the presence of 220 mM sodium chloride or 60 mM lithium chloride. Cuttings of mature transgenic leaf exhibited a marked tolerance upon treatment with 500 mM mannitol for 12 h, at which concentration wild-type counterparts were seriously damaged. These results suggested that NtC7 predominantly functions in maintenance of osmotic adjustment independently of ion homeostasis.
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