Plant Physiology Preview
Published on March 6, 2003; 10.1104/pp.102.016444
Received October 24, 2002
Returned for revision November 20, 2002
Accepted December 23, 2002
Distribution of Fucose-Containing Xyloglucans in Cell Walls of the mur1 Mutant of Arabidopsis
Glenn Freshour , Christopher P. Bonin , Wolf-Dieter Reiter , Peter Albersheim , Alan G. Darvill , and Michael G. Hahn *
The University of Georgia, Complex Carbohydrate Research Center (G.F., P.A., A.G.D., M.G.H.) and Departments of Plant Biology (M.G.H.) and Biochemistry and Molecular Biology (P.A., A.G.D.), 220 Riverbend Road, Athens, Georgia 30602-4712; and University of Connecticut, Department of Molecular and Cell Biology, Storrs, Connecticut 06269 (C.P.B., W.-D.R.)
* Corresponding author; email: hahn{at}ccrc.uga.edu.
The monoclonal antibody, CCRC-M1, which recognizes a fucose (Fuc)-containing epitope found principally in the cell wall polysaccharide xyloglucan, was used to determine the distribution of this epitope throughout the mur1 mutant of Arabidopsis. Immunofluorescent labeling of whole seedlings revealed that mur1 root hairs are stained heavily by CCRC-M1, whereas the body of the root remains unstained or only lightly stained. Immunogold labeling showed that CCRC-M1 labeling within the mur1 root is specific to particular cell walls and cell types. CCRC-M1 labels all cell walls at the apex of primary roots 2 d and older and the apices of mature lateral roots, but does not bind to cell walls in lateral root initials. Labeling with CCRC-M1 decreases in mur1 root cells that are undergoing rapid elongation growth such that, in the mature portions of primary and lateral roots, only the walls of pericycle cells and the outer walls of epidermal cells are labeled. Growth of the mutant on Fuc-containing media restores wild-type labeling, where all cell walls are labeled by the CCRC-M1 antibody. No labeling was observed in mur1 hypocotyls, shoots, or leaves; stipules are labeled. CCRC-M1 does label pollen grains within anthers and pollen tube walls. These results suggest the Fuc destined for incorporation into xyloglucan is synthesized using one or the other or both isoforms of GDP-D-mannose 4,6-dehydratase, depending on the cell type and/or developmental state of the cell.
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