Plant Physiol. Drug Metab Dispos
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Published on March 13, 2003; 10.1104/pp.102.016527

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Received October 22, 2002
Returned for revision December 2, 2002
Accepted January 30, 2003

STA11, a Chlamydomonas reinhardtii Locus Required for Normal Starch Granule Biogenesis, Encodes Disproportionating Enzyme. Further Evidence for a Function of {alpha}-1,4 Glucanotransferases during Starch Granule Biosynthesis in Green Algae

Fabrice Wattebled , Jean-Philippe Ral , David Dauvillée , Alan M. Myers , Martha G. James , Ralf Schlichting , Christoph Giersch , Steven G. Ball , and Christophe D'Hulst *

Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche Centre National de la Recherche Scientifique/Université des Sciences et Technologies Lille 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve D'Ascq cedex, France (F.W., J.-P.R., D.D., S.B., C.D.H.); Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M., M.J.); and Institut für Botanik, Technische Universität, D-64287 Darmstadt, Germany (R.S., C.G.)

* Corresponding author; email: christophe.dhulst{at}univ-lille1.fr.

In Chlamydomonas reinhardtii, the presence of a defective STA11 locus results in significantly reduced granular starch deposition displaying major modifications in shape and structure. This defect simultaneously leads to the accumulation of linear malto-oligosaccharides (MOS). The mutants of STA11 were showed to lack D-enzyme, a plant {alpha}-1,4 glucanotransferase analogous to the Escherichia coli amylomaltase. We have cloned and characterized both the cDNA and gDNA corresponding to the C. reinhardtii D-enzyme. We now report allele-specific modifications of the D-enzyme gene in the mutants of STA11. These allele-specific modifications cosegregate with the corresponding sta11 mutations, thereby demonstrating that STA11 encodes D-enzyme. MOS production and starch accumulation were investigated during day and night cycles in wild-type and mutant C. reinhardtii cells. We demonstrate that in the algae MOS are produced during starch biosynthesis and degraded during the phases of net polysaccharide catabolism.




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