Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on March 20, 2003; 10.1104/pp.102.016725

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
131/4/1566    most recent
pp.102.016725v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (32)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gao, Z.
Right arrow Articles by Loescher, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gao, Z.
Right arrow Articles by Loescher, W.
Agricola
Right arrow Articles by Gao, Z.
Right arrow Articles by Loescher, W.

Received October 25, 2002
Returned for revision November 24, 2002
Accepted January 29, 2003

Cloning, Expression, and Characterization of Sorbitol Transporters from Developing Sour Cherry Fruit and Leaf Sink Tissues

Zhifang Gao , Laurence Maurousset , Remi Lemoine , Sang-Dong Yoo , Steven van Nocker , and Wayne Loescher *

Department of Horticulture, Michigan State University, East Lansing, Michigan 48824 (Z.G., S.-D.Y., S.v.N., W.L.); and Laboratoire de Physiologie et Biochimie Végétales, Unité Mixte de Recherches 6161, Centre National de la Recherche Scientifique, Bâtiment Botanique, Université de Poitiers, 40 Avenue du Recteur Pineau, 86022 Poitiers cedex, France (L.M., R.L.)

* Corresponding author; email: loescher{at}msu.edu.

The acyclic polyol sorbitol is a primary photosynthetic product and the principal photosynthetic transport substance in many economically important members of the family Rosaceace (e.g. almond [Prunus dulcis (P. Mill.) D.A. Webber], apple [Malus pumila P. Mill.], cherry [Prunus spp.], peach [Prunus persica L. Batsch], and pear [Pyrus communis]). To understand key steps in long-distance transport and particularly partitioning and accumulation of sorbitol in sink tissues, we have cloned two sorbitol transporter genes (PcSOT1 and PcSOT2) from sour cherry (Prunus cerasus) fruit tissues that accumulate large quantities of sorbitol. Sorbitol uptake activities and other characteristics were measured by heterologous expression of PcSOT1 and PcSOT2 in yeast (Saccharomyces cerevisiae). Both genes encode proton-dependent, sorbitol-specific transporters with similar affinities (Km sorbitol of 0.81 mM for PcSOT1 and 0.64 mM for PcSOT2). Analyses of gene expression of these transporters, however, suggest different roles during leaf and fruit development. PcSOT1 is expressed throughout fruit development, but especially when growth and sorbitol accumulation rates are highest. In leaves, PcSOT1 expression is highest in young, expanding tissues, but substantially less in mature leaves. In contrast, PcSOT2 is mainly expressed only early in fruit development and not in leaves. Compositional analyses suggest that transport mediated by PcSOT1 and PcSOT2 plays a major role in sorbitol and dry matter accumulation in sour cherry fruits. Presence of these transporters and the high fruit sorbitol concentrations suggest that there is an apoplastic step during phloem unloading and accumulation in these sink tissues. Expression of PcSOT1 in young leaves before completion of the transition from sink to source is further evidence for a role in determining sink activity.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R.-C. Fan, C.-C. Peng, Y.-H. Xu, X.-F. Wang, Y. Li, Y. Shang, S.-Y. Du, R. Zhao, X.-Y. Zhang, L.-Y. Zhang, et al.
Apple Sucrose Transporter SUT1 and Sorbitol Transporter SOT6 Interact with Cytochrome b5 to Regulate Their Affinity for Substrate Sugars
Plant Physiology, August 1, 2009; 150(4): 1880 - 1901.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. J. Reidel, E. A. Rennie, V. Amiard, L. Cheng, and R. Turgeon
Phloem Loading Strategies in Three Plant Species That Transport Sugar Alcohols
Plant Physiology, March 1, 2009; 149(3): 1601 - 1608.
[Abstract] [Full Text] [PDF]

Home page
 Food Science and Technology InternationalHome page
N. Garcia-Marino, F. de la Torre, and A.J. Matilla
Organic Acids and Soluble Sugars in Edible and Nonedible Parts of Damson Plum (Prunus domestica L. subsp. insititia cv. Syriaca) Fruits During Development and Ripening
Food Science and Technology International, April 1, 2008; 14(2): 187 - 193.
[Abstract] [PDF]

Home page
 Plant CellHome page
S. Schneider, D. Beyhl, R. Hedrich, and N. Sauer
Functional and Physiological Characterization of Arabidopsis INOSITOL TRANSPORTER1, a Novel Tonoplast-Localized Transporter for myo-Inositol
PLANT CELL, April 1, 2008; 20(4): 1073 - 1087.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Juchaux-Cachau, L. Landouar-Arsivaud, J.-P. Pichaut, C. Campion, B. Porcheron, J. Jeauffre, N. Noiraud-Romy, P. Simoneau, L. Maurousset, and R. Lemoine
Characterization of AgMaT2, a Plasma Membrane Mannitol Transporter from Celery, Expressed in Phloem Cells, Including Phloem Parenchyma Cells
Plant Physiology, September 1, 2007; 145(1): 62 - 74.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Pommerrenig, F. S. Papini-Terzi, and N. Sauer
Differential Regulation of Sorbitol and Sucrose Loading into the Phloem of Plantago major in Response to Salt Stress
Plant Physiology, June 1, 2007; 144(2): 1029 - 1038.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
C. Conde, P. Silva, A. Agasse, R. Lemoine, S. Delrot, R. Tavares, and H. Geros
Utilization and Transport of Mannitol in Olea europaea and Implications for Salt Stress Tolerance
Plant Cell Physiol., January 1, 2007; 48(1): 42 - 53.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Teo, Y. Suzuki, S. L. Uratsu, B. Lampinen, N. Ormonde, W. K. Hu, T. M. DeJong, and A. M. Dandekar
Colloquium Paper: Silencing leaf sorbitol synthesis alters long-distance partitioning and apple fruit quality
PNAS, December 5, 2006; 103(49): 18842 - 18847.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Hemmerlin, D. Tritsch, M. Hartmann, K. Pacaud, J.-F. Hoeffler, A. van Dorsselaer, M. Rohmer, and T. J. Bach
A Cytosolic Arabidopsis D-Xylulose Kinase Catalyzes the Phosphorylation of 1-Deoxy-D-Xylulose into a Precursor of the Plastidial Isoprenoid Pathway
Plant Physiology, October 1, 2006; 142(2): 441 - 457.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Conde, A. Agasse, D. Glissant, R. Tavares, H. Geros, and S. Delrot
Pathways of Glucose Regulation of Monosaccharide Transport in Grape Cells
Plant Physiology, August 1, 2006; 141(4): 1563 - 1577.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Schneider, A. Schneidereit, K. R. Konrad, M.-R. Hajirezaei, M. Gramann, R. Hedrich, and N. Sauer
Arabidopsis INOSITOL TRANSPORTER4 Mediates High-Affinity H+ Symport of Myoinositol across the Plasma Membrane
Plant Physiology, June 1, 2006; 141(2): 565 - 577.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Vignault, M. Vachaud, B. Cakir, D. Glissant, F. Dedaldechamp, M. Buttner, R. Atanassova, P. Fleurat-Lessard, R. Lemoine, and S. Delrot
VvHT1 encodes a monosaccharide transporter expressed in the conducting complex of the grape berry phloem
J. Exp. Bot., May 1, 2005; 56(415): 1409 - 1418.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. Ferreira, F. van Voorst, A. Martins, L. Neves, R. Oliveira, M. C. Kielland-Brandt, C. Lucas, and A. Brandt
A Member of the Sugar Transporter Family, Stl1p Is the Glycerol/H+ Symporter in Saccharomyces cerevisiae
Mol. Biol. Cell, April 1, 2005; 16(4): 2068 - 2076.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Reinders, J. A. Panshyshyn, and J. M. Ward
Analysis of Transport Activity of Arabidopsis Sugar Alcohol Permease Homolog AtPLT5
J. Biol. Chem., January 14, 2005; 280(2): 1594 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y.-S. Klepek, D. Geiger, R. Stadler, F. Klebl, L. Landouar-Arsivaud, R. Lemoine, R. Hedrich, and N. Sauer
Arabidopsis POLYOL TRANSPORTER5, a New Member of the Monosaccharide Transporter-Like Superfamily, Mediates H+-Symport of Numerous Substrates, Including myo-Inositol, Glycerol, and Ribose
PLANT CELL, January 1, 2005; 17(1): 204 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. Watari, Y. Kobae, S. Yamaki, K. Yamada, K. Toyofuku, T. Tabuchi, and K. Shiratake
Identification of Sorbitol Transporters Expressed in the Phloem of Apple Source Leaves
Plant Cell Physiol., August 15, 2004; 45(8): 1032 - 1041.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L.-Y. Zhang, Y.-B. Peng, S. Pelleschi-Travier, Y. Fan, Y.-F. Lu, Y.-M. Lu, X.-P. Gao, Y.-Y. Shen, S. Delrot, and D.-P. Zhang
Evidence for Apoplasmic Phloem Unloading in Developing Apple Fruit
Plant Physiology, May 1, 2004; 135(1): 574 - 586.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Ramsperger-Gleixner, D. Geiger, R. Hedrich, and N. Sauer
Differential Expression of Sucrose Transporter and Polyol Transporter Genes during Maturation of Common Plantain Companion Cells
Plant Physiology, January 1, 2004; 134(1): 147 - 160.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists