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Published on June 5, 2003; 10.1104/pp.102.019794

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Received December 27, 2002
Returned for revision January 24, 2003
Accepted April 4, 2003

Ectopic Expression of a Horseradish Peroxidase Enhances Growth Rate and Increases Oxidative Stress Resistance in Hybrid Aspen

Akiyoshi Kawaoka *, Etsuko Matsunaga , Saori Endo , Shinkichi Kondo , Kazuya Yoshida , Atsuhiko Shinmyo , and Hiroyasu Ebinuma

Pulp and Paper Research Laboratory, Nippon Paper Industries Co. Ltd., 5-21-1 Oji, Kita-ku, Tokyo 114-0002, Japan (A.K., E.M., S.E., H.E.); Jujo Research Co. Ltd., 5-5-24 Oji, Kita-ku, Tokyo 114-0002, Japan (S.K.); and Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan (K.Y., A.S.)

* Corresponding author; email: akiyoshi.kawaoka{at}nifty.ne.jp.

We previously demonstrated that overexpression of the horseradish (Armoracia rusticana) peroxidase prxC1a gene stimulated the growth rate of tobacco (Nicotiana tabacum) plants. Here, the cauliflower mosaic virus 35S::prxC1a construct was introduced into hybrid aspen (Populus sieboldii x Populus grandidentata). The growth rate of these transformed hybrid aspen plants was substantially increased under greenhouse conditions. The average stem length of transformed plants was 25% greater than that of control plants. There was no other obvious phenotypic difference between the transformed and control plants. Fast-growing transformed hybrid aspen showed high levels of expression of prxC1a and had elevated peroxidase activities toward guaiacol and ascorbate. However, there was no increase of the endogenous class I ascorbate peroxidase activities in the transformed plants by separate assay and activity staining of native polyacrylamide gel electrophoresis. Furthermore, calli derived from the transformed hybrid aspen grew faster than those from control plants and were resistant to the oxidative stress imposed by hydrogen peroxide. Therefore, enhanced peroxidase activity affects plant growth rate and oxidative stress resistance.




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