Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on July 10, 2003; 10.1104/pp.103.020008

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
132/4/1840    most recent
pp.103.020008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (58)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dammann, C.
Right arrow Articles by Harper, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dammann, C.
Right arrow Articles by Harper, J. F.
Agricola
Right arrow Articles by Dammann, C.
Right arrow Articles by Harper, J. F.

Received January 3, 2003
Returned for revision February 5, 2003
Accepted April 21, 2003

Subcellular Targeting of Nine Calcium-Dependent Protein Kinase Isoforms from Arabidopsis

Christian Dammann , Audrey Ichida , Bimei Hong , Shawn M. Romanowsky , Estelle M. Hrabak , Alice C. Harmon , Barbara G. Pickard , and Jeffrey F. Harper *

Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037 (C.D., B.H., S.M.R., J.F.H.); Biology Department, Washington University, St. Louis, Missouri 63130-4899 (A.I., B.G.P.); Department of Plant Biology, University of New Hampshire, 46 College Road, Durham, New Hampshire 03824 (E.M.H.); and Botany Department, University of Florida, Gainesville, Florida 32611-8526 (A.C.H.)

* Corresponding author; email: Harper{at}Scripps.edu.

Calcium-dependent protein kinases (CDPKs) are specific to plants and some protists. Their activation by calcium makes them important switches for the transduction of intracellular calcium signals. Here, we identify the subcellular targeting potentials for nine CDPK isoforms from Arabidopsis, as determined by expression of green fluorescent protein (GFP) fusions in transgenic plants. Subcellular locations were determined by fluorescence microscopy in cells near the root tip. Isoforms AtCPK3-GFP and AtCPK4-GFP showed a nuclear and cytosolic distribution similar to that of free GFP. Membrane fractionation experiments confirmed that these isoforms were primarily soluble. A membrane association was observed for AtCPKs 1, 7, 8, 9, 16, 21, and 28, based on imaging and membrane fractionation experiments. This correlates with the presence of potential N-terminal acylation sites, consistent with acylation as an important factor in membrane association. All but one of the membrane-associated isoforms targeted exclusively to the plasma membrane. The exception was AtCPK1-GFP, which targeted to peroxisomes, as determined by covisualization with a peroxisome marker. Peroxisome targeting of AtCPK1-GFP was disrupted by a deletion of two potential N-terminal acylation sites. The observation of a peroxisome-located CDPK suggests a mechanism for calcium regulation of peroxisomal functions involved in oxidative stress and lipid metabolism.




This article has been cited by other articles:


Home page
 J Exp BotHome page
X. Wang, Q. Li, X. Niu, H. Chen, L. Xu, and C. Qi
Characterization of a canola C2 domain gene that interacts with PG, an effector of the necrotrophic fungus Sclerotinia sclerotiorum
J. Exp. Bot., July 1, 2009; 60(9): 2613 - 2620.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
M. C. Kim, W. S. Chung, D.-J. Yun, and M. J. Cho
Calcium and Calmodulin-Mediated Regulation of Gene Expression in Plants
Mol Plant, January 6, 2009; (2009) ssn091v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Arai, M. Hayashi, and M. Nishimura
Proteomic Identification and Characterization of a Novel Peroxisomal Adenine Nucleotide Transporter Supplying ATP for Fatty Acid {beta}-Oxidation in Soybean and Arabidopsis
PLANT CELL, December 1, 2008; 20(12): 3227 - 3240.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Ma and S. Reumann
Improved prediction of peroxisomal PTS1 proteins from genome sequences based on experimental subcellular targeting analyses as exemplified for protein kinases from Arabidopsis
J. Exp. Bot., October 1, 2008; 59(13): 3767 - 3779.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. Marmagne, M. Ferro, T. Meinnel, C. Bruley, L. Kuhn, J. Garin, H. Barbier-Brygoo, and G. Ephritikhine
A High Content in Lipid-modified Peripheral Proteins and Integral Receptor Kinases Features in the Arabidopsis Plasma Membrane Proteome
Mol. Cell. Proteomics, November 1, 2007; 6(11): 1980 - 1996.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S.-Y. Zhu, X.-C. Yu, X.-J. Wang, R. Zhao, Y. Li, R.-C. Fan, Y. Shang, S.-Y. Du, X.-F. Wang, F.-Q. Wu, et al.
Two Calcium-Dependent Protein Kinases, CPK4 and CPK11, Regulate Abscisic Acid Signal Transduction in Arabidopsis
PLANT CELL, October 1, 2007; 19(10): 3019 - 3036.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Reumann, L. Babujee, C. Ma, S. Wienkoop, T. Siemsen, G. E. Antonicelli, N. Rasche, F. Luder, W. Weckwerth, and O. Jahn
Proteome Analysis of Arabidopsis Leaf Peroxisomes Reveals Novel Targeting Peptides, Metabolic Pathways, and Defense Mechanisms
PLANT CELL, October 1, 2007; 19(10): 3170 - 3193.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. N. Martin, P. H. Saladores, E. Lambert, A. O. Hudson, and T. Leustek
Localization of Members of the {gamma}-Glutamyl Transpeptidase Family Identifies Sites of Glutathione and Glutathione S-Conjugate Hydrolysis
Plant Physiology, August 1, 2007; 144(4): 1715 - 1732.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Ide, N. Nagasaki, R. Tomioka, M. Suito, T. Kamiya, and M. Maeshima
Molecular properties of a novel, hydrophilic cation-binding protein associated with the plasma membrane
J. Exp. Bot., March 1, 2007; 58(5): 1173 - 1183.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Kobayashi, I. Ohura, K. Kawakita, N. Yokota, M. Fujiwara, K. Shimamoto, N. Doke, and H. Yoshioka
Calcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH Oxidase
PLANT CELL, March 1, 2007; 19(3): 1065 - 1080.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Ide, R. Tomioka, Y. Ouchi, T. Kamiya, and M. Maeshima
Transcriptional Induction of Two Genes for CCaPs, Novel Cytosolic Proteins, in Arabidopsis thaliana in the Dark
Plant Cell Physiol., January 1, 2007; 48(1): 54 - 65.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Liu, B.-C. Yoo, J.-Y. Lee, W. Pan, and A. C. Harmon
Calcium-regulated Phosphorylation of Soybean Serine Acetyltransferase in Response to Oxidative Stress
J. Biol. Chem., September 15, 2006; 281(37): 27405 - 27415.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
C. J. Nelson, A. D. Hegeman, A. C. Harms, and M. R. Sussman
A Quantitative Analysis of Arabidopsis Plasma Membrane Using Trypsin-catalyzed 18O Labeling
Mol. Cell. Proteomics, August 1, 2006; 5(8): 1382 - 1395.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Raichaudhuri, R. Bhattacharyya, S. Chaudhuri, P. Chakrabarti, and M. DasGupta
Domain Analysis of a Groundnut Calcium-dependent Protein Kinase: NUCLEAR LOCALIZATION SEQUENCE IN THE JUNCTION DOMAIN IS COUPLED WITH NONCONSENSUS CALCIUM BINDING DOMAINS
J. Biol. Chem., April 14, 2006; 281(15): 10399 - 10409.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. M. Yoon, P. E. Dowd, S. Gilroy, and A. G. McCubbin
Calcium-Dependent Protein Kinase Isoforms in Petunia Have Distinct Functions in Pollen Tube Growth, Including Regulating Polarity
PLANT CELL, April 1, 2006; 18(4): 867 - 878.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X.-C. Yu, M.-J. Li, G.-F. Gao, H.-Z. Feng, X.-Q. Geng, C.-C. Peng, S.-Y. Zhu, X.-J. Wang, Y.-Y. Shen, and D.-P. Zhang
Abscisic Acid Stimulates a Calcium-Dependent Protein Kinase in Grape Berry
Plant Physiology, February 1, 2006; 140(2): 558 - 579.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Szczegielniak, M. Klimecka, A. Liwosz, A. Ciesielski, S. Kaczanowski, G. Dobrowolska, A. C. Harmon, and G. Muszynska
A Wound-Responsive and Phospholipid-Regulated Maize Calcium-Dependent Protein Kinase
Plant Physiology, December 1, 2005; 139(4): 1970 - 1983.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Asano, N. Tanaka, G. Yang, N. Hayashi, and S. Komatsu
Genome-wide Identification of the Rice Calcium-dependent Protein Kinase and its Closely Related Kinase Gene Families: Comprehensive Analysis of the CDPKs Gene Family in Rice
Plant Cell Physiol., February 1, 2005; 46(2): 356 - 366.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Leclercq, B. Ranty, M.-T. Sanchez-Ballesta, Z. Li, B. Jones, A. Jauneau, J.-C. Pech, A. Latche, R. Ranjeva, and M. Bouzayen
Molecular and biochemical characterization of LeCRK1, a ripening-associated tomato CDPK-related kinase
J. Exp. Bot., January 1, 2005; 56(409): 25 - 35.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Alexandersson, G. Saalbach, C. Larsson, and P. Kjellbom
Arabidopsis Plasma Membrane Proteomics Identifies Components of Transport, Signal Transduction and Membrane Trafficking
Plant Cell Physiol., November 15, 2004; 45(11): 1543 - 1556.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Reumann, C. Ma, S. Lemke, and L. Babujee
AraPerox. A Database of Putative Arabidopsis Proteins from Plant Peroxisomes
Plant Physiology, September 1, 2004; 136(1): 2587 - 2608.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. J. Corpas, J. B. Barroso, A. Carreras, M. Quiros, A. M. Leon, M. C. Romero-Puertas, F. J. Esteban, R. Valderrama, J. M. Palma, L. M. Sandalio, et al.
Cellular and Subcellular Localization of Endogenous Nitric Oxide in Young and Senescent Pea Plants
Plant Physiology, September 1, 2004; 136(1): 2722 - 2733.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. W. Chehab, O. R. Patharkar, A. D. Hegeman, T. Taybi, and J. C. Cushman
Autophosphorylation and Subcellular Localization Dynamics of a Salt- and Water Deficit-Induced Calcium-Dependent Protein Kinase from Ice Plant
Plant Physiology, July 1, 2004; 135(3): 1430 - 1446.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists