Plant Physiology Preview
Published on June 5, 2003; 10.1104/pp.103.020354
Received January 10, 2003
Returned for revision February 21, 2003
Accepted March 20, 2003
Activation Tagging of a Dominant Gibberellin Catabolism Gene (GA 2-oxidase) from Poplar That Regulates Tree Stature
Victor B. Busov , Richard Meilan , David W. Pearce , Caiping Ma , Stewart B. Rood , and Steven H. Strauss *
Department of Forest Science, Oregon State University, Corvallis, Oregon 97331-5752 (V.B.B., R.M., C.M., S.H.S.); and University of Lethbridge, Department of Biological Sciences, Lethbridge, Alberta, Canada T1K 3M4 (D.W.P., S.B.R.)
* Corresponding author; email: Steve.Strauss{at}orst.edu.
We identified a dwarf transgenic hybrid poplar (Populus tremula x Populus alba) after screening of 627 independent activation-tagged transgenic lines in tissue culture, greenhouse, and field environments. The cause of the phenotype was a hyperactivated gene encoding GA 2-oxidase (GA2ox), the major gibberellin (GA) catabolic enzyme in plants. The mutation resulted from insertion of a strong transcriptional enhancer near the transcription start site. Overexpression of the poplar GA2ox gene (PtaGA2ox1) caused hyperaccumulation of mRNA transcripts, quantitative shifts in the spectrum of GAs, and similarity in phenotype to transgenic poplars that overexpress a bean (Phaseolus coccineus) GA2ox gene. The poplar PtaGA2ox1 sequence was most closely related to PsGA2ox2 from pea (Pisum sativum) and two poorly known GA2oxs from Arabidopsis (AtGA2ox4 and AtGA2ox5). The dwarf phenotype was reversible through gibberellic acid application to the shoot apex. Transgenic approaches to producing semidwarf trees for use in arboriculture, horticulture, and forestry could have significant economic and environmental benefits, including altered fiber and fruit production, greater ease of management, and reduced risk of spread in wild populations.
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