Plant Physiology Preview
Published on October 2, 2003; 10.1104/pp.103.021527
Received February 2, 2003
Returned for revision March 3, 2003
Accepted July 10, 2003
Starch-Branching Enzyme I-Deficient Mutation Specifically Affects the Structure and Properties of Starch in Rice Endosperm
Hikaru Satoh *, Aiko Nishi , Kazuhiro Yamashita , Yoko Takemoto , Yasumasa Tanaka , Yuko Hosaka , Aya Sakurai , Naoko Fujita , and Yasunori Nakamura
Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan (H.S., A.N., K.Y., Y. Takemoto, Y. Tanaka); Core Research for Evolutional Science and Technology, Japan Science and Technology (Y.H., A.S., N.F., Y.N.); and Faculty of Bioresource Science, Akita Prefectural University, Shimoshinjo-Nakano, Akita-City 010-0195, Japan (N.F., Y.N.)
* Corresponding author; email: hsatoh{at}agr.kyushu-u.ac.jp.
We have isolated a starch mutant that was deficient in starch-branching enzyme I (BEI) from the endosperm mutant stocks of rice (Oryza sativa) induced by the treatment of fertilized egg cells with N-methyl-N-nitrosourea. The deficiency of BEI in this mutant was controlled by a single recessive gene, tentatively designated as starch-branching enzyme mutant 1 (sbe1). The mutant endosperm exhibited the normal phenotype and contained the same amount of starch as the wild type. However, the mutation apparently altered the fine structure of amylopectin. The mutant amylopectin was characterized by significant decrease in both long chains with degree of polymerization (DP)  37 and short chains with DP 12 to 21, marked increase in short chains with DP  10 (A chains), and slight increase in intermediate chains with DP 24 to 34, suggesting that BEI specifically synthesizes B1 and B2-3 chains. The endosperm starch from the sbe1 mutant had a lower onset concentration for urea gelatinization and a lower onset temperature for thermo-gelatinization compared with the wild type, indicating that the genetic modification of amylopectin fine structure is responsible for changes in physicochemical properties of sbe1 starch.
This article has been cited by other articles:
|
|
|
|
 
H.-L. Tsai, W.-L. Lue, K.-J. Lu, M.-H. Hsieh, S.-M. Wang, and J. Chen
Starch Synthesis in Arabidopsis Is Achieved by Spatial Cotranscription of Core Starch Metabolism Genes
Plant Physiology,
November 1, 2009;
151(3):
1582 - 1595.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Sawada, P. B. Francisco Jr, S. Aihara, Y. Utsumi, M. Yoshida, Y. Oyama, M. Tsuzuki, H. Satoh, and Y. Nakamura
Chlorella Starch Branching Enzyme II (BEII) Can Complement the Function of BEIIb in Rice Endosperm
Plant Cell Physiol.,
June 1, 2009;
50(6):
1062 - 1074.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-y. Wu, A. Trieu, P. Radhakrishnan, S. F. Kwok, S. Harris, K. Zhang, J. Wang, J. Wan, H. Zhai, S. Takatsuto, et al.
Brassinosteroids Regulate Grain Filling in Rice
PLANT CELL,
August 1, 2008;
20(8):
2130 - 2145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Satoh, K. Shibahara, T. Tokunaga, A. Nishi, M. Tasaki, S.-K. Hwang, T. W. Okita, N. Kaneko, N. Fujita, M. Yoshida, et al.
Mutation of the Plastidial {alpha}-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm
PLANT CELL,
July 1, 2008;
20(7):
1833 - 1849.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. S Dennis, J. Ellis, A. Green, D. Llewellyn, M. Morell, L. Tabe, and W.J Peacock
Genetic contributions to agricultural sustainability
Phil Trans R Soc B,
February 12, 2008;
363(1491):
591 - 609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Dumez, F. Wattebled, D. Dauvillee, D. Delvalle, V. Planchot, S. G. Ball, and C. D'Hulst
Mutants of Arabidopsis Lacking Starch Branching Enzyme II Substitute Plastidial Starch Synthesis by Cytoplasmic Maltose Accumulation
PLANT CELL,
October 1, 2006;
18(10):
2694 - 2709.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ohdan, P. B. Francisco Jr, T. Sawada, T. Hirose, T. Terao, H. Satoh, and Y. Nakamura
Expression profiling of genes involved in starch synthesis in sink and source organs of rice
J. Exp. Bot.,
December 1, 2005;
56(422):
3229 - 3244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yao, D. B. Thompson, and M. J. Guiltinan
Maize Starch-Branching Enzyme Isoforms and Amylopectin Structure. In the Absence of Starch-Branching Enzyme IIb, the Further Absence of Starch-Branching Enzyme Ia Leads to Increased Branching
Plant Physiology,
November 1, 2004;
136(3):
3515 - 3523.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. J. Tetlow, M. K. Morell, and M. J. Emes
Recent developments in understanding the regulation of starch metabolism in higher plants
J. Exp. Bot.,
October 1, 2004;
55(406):
2131 - 2145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Palaisa, M. Morgante, S. Tingey, and A. Rafalski
Long-range patterns of diversity and linkage disequilibrium surrounding the maize Y1 gene are indicative of an asymmetric selective sweep
PNAS,
June 29, 2004;
101(26):
9885 - 9890.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. J. Tetlow, R. Wait, Z. Lu, R. Akkasaeng, C. G. Bowsher, S. Esposito, B. Kosar-Hashemi, M. K. Morell, and M. J. Emes
Protein Phosphorylation in Amyloplasts Regulates Starch Branching Enzyme Activity and Protein-Protein Interactions
PLANT CELL,
March 1, 2004;
16(3):
694 - 708.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
