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Published on August 14, 2003; 10.1104/pp.103.022723

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Received February 26, 2003
Returned for revision April 17, 2003
Accepted June 9, 2003

Insect Attack and Wounding Induce Traumatic Resin Duct Development and Gene Expression of (-)-Pinene Synthase in Sitka Spruce

S. Ashley Byun McKay , William L. Hunter , Kimberley-Ann Godard , Shawn X. Wang , Diane M. Martin , Jörg Bohlmann , and Aine L. Plant *

Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada (S.A.B.M., W.L.H., S.X.W., A.L.P.); and Biotechnology Laboratory, Departments of Botany and Forest Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada (K.-A.G., D.M.M., J.B.)

* Corresponding author; email: Aine_Plant{at}sfu.ca.

Conifers possess inducible terpenoid defense systems. These systems are associated with the formation of traumatic resin ducts (TRD) and are underpinned by enhanced gene expression and activity of terpene synthases (TPS), enzymes responsible for oleoresin formation. We first determined that Sitka spruce (Picea sitchensis [Bong.] Carriere) had the capacity for TRD formation by mechanically wounding representative trees. We then proceeded to investigate whether the white pine weevil (Pissodes strobi Peck.), a stem-boring insect, can influence the expression of genes encoding monoterpene synthases (mono-tps) in Sitka spruce. We went on to compare this response with the effects of a simulated insect attack by drill wounding. A significant increase in mono-tps transcript level was observed in the leaders of lateral branches of weevil-attacked and mechanically wounded trees. In this study, weevils induced a more rapid enhancement of mono-tps gene expression. A full-length Sitka spruce mono-tps cDNA (PsTPS2) was isolated, expressed in Escherichia coli, and functionally identified as (-)-pinene synthase. The recombinant (-)-pinene synthase catalyzes the formation of (-)-{alpha}-pinene and (-)-{beta}-pinene, both of which are known constituents of stem oleoresin in Sitka spruce and increase in abundance after weevil attack. These data suggest that increased (-)-pinene synthase gene expression is an important element of the direct defense system deployed in Sitka spruce after insect attack.




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