Plant Physiology Preview
Published on October 2, 2003; 10.1104/pp.103.025122
Received April 8, 2003
Returned for revision June 17, 2003
Accepted July 2, 2003
Overexpression of the FRO2 Ferric Chelate Reductase Confers Tolerance to Growth on Low Iron and Uncovers Posttranscriptional Control
Erin L. Connolly *, Nathan H. Campbell , Natasha Grotz , Charis L. Prichard , and Mary Lou Guerinot
Department of Biological Sciences, Coker Life Sciences, University of South Carolina, Columbia, South Carolina 29208 (E.L.C., N.H.C., C.L.P.); and Department of Biological Sciences, 6044 Gilman, Dartmouth College, Hanover, New Hampshire 03755 (N.G., M.L.G.)
* Corresponding author; email: erinc{at}biol.sc.edu.
The Arabidopsis FRO2 gene encodes the low-iron-inducible ferric chelate reductase responsible for reduction of iron at the root surface. Here, we report that FRO2 and IRT1, the major transporter responsible for high-affinity iron uptake from the soil, are coordinately regulated at both the transcriptional and posttranscriptional levels. FRO2 and IRT1 are induced together following the imposition of iron starvation and are coordinately repressed following iron resupply. Steady-state mRNA levels of FRO2 and IRT1 are also coordinately regulated by zinc and cadmium. Like IRT1, FRO2 mRNA is detected in the epidermal cells of roots, consistent with its proposed role in iron uptake from the soil. FRO2 mRNA is detected at high levels in the roots and shoots of 35S-FRO2 transgenic plants. However, ferric chelate reductase activity is only elevated in the 35S-FRO2 plants under conditions of iron deficiency, indicating that FRO2 is subject to posttranscriptional regulation, as shown previously for IRT1. Finally, the 35S-FRO2 plants grow better on low iron as compared with wild-type plants, supporting the idea that reduction of ferric iron to ferrous iron is the rate-limiting step in iron uptake.
This article has been cited by other articles:
|
|
|
|
 
L. Kerkeb, I. Mukherjee, I. Chatterjee, B. Lahner, D. E. Salt, and E. L. Connolly
Iron-Induced Turnover of the Arabidopsis IRON-REGULATED TRANSPORTER1 Metal Transporter Requires Lysine Residues
Plant Physiology,
April 1, 2008;
146(4):
1964 - 1973.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Santi and W. Schmidt
Laser microdissection-assisted analysis of the functional fate of iron deficiency-induced root hairs in cucumber
J. Exp. Bot.,
March 2, 2008;
(2008)
erm351v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Allen, J. A. del Campo, J. Kropat, and S. S. Merchant
FEA1, FEA2, and FRE1, Encoding Two Homologous Secreted Proteins and a Candidate Ferrireductase, Are Expressed Coordinately with FOX1 and FTR1 in Iron-Deficient Chlamydomonas reinhardtii
Eukaryot. Cell,
October 1, 2007;
6(10):
1841 - 1852.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ishimaru, H. Masuda, M. Suzuki, K. Bashir, M. Takahashi, H. Nakanishi, S. Mori, and N. K. Nishizawa
Overexpression of the OsZIP4 zinc transporter confers disarrangement of zinc distribution in rice plants
J. Exp. Bot.,
August 1, 2007;
58(11):
2909 - 2915.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ishimaru, S. Kim, T. Tsukamoto, H. Oki, T. Kobayashi, S. Watanabe, S. Matsuhashi, M. Takahashi, H. Nakanishi, S. Mori, et al.
From the Cover: Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil
PNAS,
May 1, 2007;
104(18):
7373 - 7378.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Guerinot
It's elementary: Enhancing Fe3+ reduction improves rice yields
PNAS,
May 1, 2007;
104(18):
7311 - 7312.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lucena, B. M. Waters, F. J. Romera, M. J. Garcia, M. Morales, E. Alcantara, and R. Perez-Vicente
Ethylene could influence ferric reductase, iron transporter, and H+-ATPase gene expression by affecting FER (or FER-like) gene activity
J. Exp. Bot.,
December 1, 2006;
57(15):
4145 - 4154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Wu, L. Li, J. Du, Y. Yuan, X. Cheng, and H.-Q. Ling
Molecular and Biochemical Characterization of the Fe(III) Chelate Reductase Gene Family in Arabidopsis thaliana
Plant Cell Physiol.,
September 1, 2005;
46(9):
1505 - 1514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Schaaf, A. Schikora, J. Haberle, G. Vert, U. Ludewig, J.-F. Briat, C. Curie, and N. von Wiren
A Putative Function for the Arabidopsis Fe-Phytosiderophore Transporter Homolog AtYSL2 in Fe and Zn Homeostasis
Plant Cell Physiol.,
May 1, 2005;
46(5):
762 - 774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Brumbarova and P. Bauer
Iron-Mediated Control of the Basic Helix-Loop-Helix Protein FER, a Regulator of Iron Uptake in Tomato
Plant Physiology,
March 1, 2005;
137(3):
1018 - 1026.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. P. Colangelo and M. L. Guerinot
The Essential Basic Helix-Loop-Helix Protein FIT1 Is Required for the Iron Deficiency Response
PLANT CELL,
December 1, 2004;
16(12):
3400 - 3412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
