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Published on September 25, 2003; 10.1104/pp.103.026138

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Received April 30, 2003
Returned for revision June 16, 2003
Accepted July 20, 2003

The Role of Homogentisate Phytyltransferase and Other Tocopherol Pathway Enzymes in the Regulation of Tocopherol Synthesis during Abiotic Stress

Eva Collakova and Dean DellaPenna *

Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing,Michigan 48824

* Corresponding author; email: dellapen{at}msu.edu.

Tocopherols are amphipathic antioxidants synthesized exclusively by photosynthetic organisms. Tocopherol levels change significantly during plant growth and development and in response to stress, likely as a consequence of the altered expression of pathway-related genes. Homogentisate phytyltransferase (HPT) is a key enzyme limiting tocopherol biosynthesis in unstressed Arabidopsis leaves (E. Collakova, D. DellaPenna [2003] Plant Physiol 131: 632-642). Wild-type and transgenic Arabidopsis plants constitutively overexpressing HPT (35S::HPT1) were subjected to a combination of abiotic stresses for up to 15 d and tocopherol levels, composition, and expression of several tocopherol pathway-related genes were determined. Abiotic stress resulted in an 18- and 8-fold increase in total tocopherol content in wild-type and 35S::HPT1 leaves, respectively, with tocopherol levels in 35S::HPT1 being 2- to 4-fold higher than wild type at all experimental time points. Increased total tocopherol levels correlated with elevated HPT mRNA levels and HPT specific activity in 35S::HPT1 and wild-type leaves, suggesting that HPT activity limits total tocopherol synthesis during abiotic stress. In addition, substrate availability and expression of pathway enzymes before HPT also contribute to increased tocopherol synthesis during stress. The accumulation of high levels of {beta}-, {gamma}-, and {delta}-tocopherols in stressed tissues suggested that the methylation of phytylquinol and tocopherol intermediates limit {alpha}-tocopherol synthesis. Overexpression of {gamma}-tocopherol methyltransferase in the 35S::HPT1 background resulted in nearly complete conversion of {gamma}- and {delta}-tocopherols to {alpha}- and {beta}-tocopherols, respectively, indicating that {gamma}-tocopherol methyltransferase activity limits {alpha}-tocopherol synthesis in stressed leaves.




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