Plant Physiology Preview
Published on August 7, 2003; 10.1104/pp.103.026674
Received May 9, 2003
Returned for revision June 10, 2003
Accepted June 17, 2003
Functional Characterization and Expression Analyses of the Glucose-Specific AtSTP9 Monosaccharide Transporter in Pollen of Arabidopsis
Alexander Schneidereit , Joachim Scholz-Starke , and Michael Büttner *
Molekulare Pflanzenphysiologie, Universität Erlangen-Nürnberg, Staudtstrasse 5, D-91058 Erlangen, Germany
* Corresponding author; email: mbuettne{at}biologie.uni-erlangen.de.
A genomic clone and the corresponding cDNA of a new Arabidopsis monosaccharide transporter AtSTP9 were isolated. Transport analysis of the expressed protein in yeast showed that AtSTP9 is an energy-dependent, uncoupler-sensitive, high-affinity monosaccharide transporter with a Km for glucose in the micromolar range. In contrast to all previously characterized monosaccharide transporters, AtSTP9 shows an unusual specificity for glucose. Reverse transcriptase-polymerase chain reaction analyses revealed that AtSTP9 is exclusively expressed in flowers, and a more detailed approach using AtSTP9 promoter/reporter plants clearly showed that AtSTP9 expression is restricted to the male gametophyte. AtSTP9 expression is not found in other floral organs or vegetative tissues. Further localization on the cellular level using a specific antibody revealed that in contrast to the early accumulation of AtSTP9 transcripts in young pollen, the AtSTP9 protein is only found weakly in mature pollen but is most prominent in germinating pollen tubes. This preloading of pollen with mRNAs has been described for genes that are essential for pollen germination and/or pollen tube growth. The pollen-specific expression found for AtSTP9 is also observed for other sugar transporters and indicates that pollen development and germination require a highly regulated supply of sugars.
This article has been cited by other articles:
|
|
|
|
 
L. Yuan, L. Graff, D. Loque, S. Kojima, Y. N. Tsuchiya, H. Takahashi, and N. von Wiren
AtAMT1;4, a Pollen-Specific High-Affinity Ammonium Transporter of the Plasma Membrane in Arabidopsis
Plant Cell Physiol.,
January 1, 2009;
50(1):
13 - 25.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wirth, F. Chopin, V. Santoni, G. Viennois, P. Tillard, A. Krapp, L. Lejay, F. Daniel-Vedele, and A. Gojon
Regulation of Root Nitrate Uptake at the NRT2.1 Protein Level in Arabidopsis thaliana
J. Biol. Chem.,
August 10, 2007;
282(32):
23541 - 23552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Footitt, D. Dietrich, A. Fait, A. R. Fernie, M. J. Holdsworth, A. Baker, and F. L. Theodoulou
The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis
Plant Physiology,
July 1, 2007;
144(3):
1467 - 1480.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Hayes, C. Davies, and I. B. Dry
Isolation, functional characterization, and expression analysis of grapevine (Vitis vinifera L.) hexose transporters: differential roles in sink and source tissues
J. Exp. Bot.,
June 1, 2007;
58(8):
1985 - 1997.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Aluri and M. Buttner
Identification and functional expression of the Arabidopsis thaliana vacuolar glucose transporter 1 and its role in seed germination and flowering
PNAS,
February 13, 2007;
104(7):
2537 - 2542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Drakakaki, O. Zabotina, I. Delgado, S. Robert, K. Keegstra, and N. Raikhel
Arabidopsis Reversibly Glycosylated Polypeptides 1 and 2 Are Essential for Pollen Development
Plant Physiology,
December 1, 2006;
142(4):
1480 - 1492.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. X. Wang, R. R. Weerasinghe, T. D. Perdue, N. G. Cakmakci, J. P. Taylor, W. F. Marzluff, and A. M. Jones
A Golgi-localized Hexose Transporter Is Involved in Heterotrimeric G Protein-mediated Early Development in Arabidopsis
Mol. Biol. Cell,
October 1, 2006;
17(10):
4257 - 4269.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Etxeberria, P. Gonzalez, P. Tomlinson, and J. Pozueta-Romero
Existence of two parallel mechanisms for glucose uptake in heterotrophic plant cells
J. Exp. Bot.,
July 1, 2005;
56(417):
1905 - 1912.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Vignault, M. Vachaud, B. Cakir, D. Glissant, F. Dedaldechamp, M. Buttner, R. Atanassova, P. Fleurat-Lessard, R. Lemoine, and S. Delrot
VvHT1 encodes a monosaccharide transporter expressed in the conducting complex of the grape berry phloem
J. Exp. Bot.,
May 1, 2005;
56(415):
1409 - 1418.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hoth, A. Schneidereit, C. Lauterbach, J. Scholz-Starke, and N. Sauer
Nematode Infection Triggers the de Novo Formation of Unloading Phloem That Allows Macromolecular Trafficking of Green Fluorescent Protein into Syncytia
Plant Physiology,
May 1, 2005;
138(1):
383 - 392.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Reinders, J. A. Panshyshyn, and J. M. Ward
Analysis of Transport Activity of Arabidopsis Sugar Alcohol Permease Homolog AtPLT5
J. Biol. Chem.,
January 14, 2005;
280(2):
1594 - 1602.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Meyer, C. Lauterbach, M. Niedermeier, I. Barth, R. D. Sjolund, and N. Sauer
Wounding Enhances Expression of AtSUC3, a Sucrose Transporter from Arabidopsis Sieve Elements and Sink Tissues
Plant Physiology,
February 1, 2004;
134(2):
684 - 693.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
