Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 9, 2003; 10.1104/pp.103.026716

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
133/3/1322    most recent
pp.103.026716v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (44)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carrari, F.
Right arrow Articles by Fernie, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carrari, F.
Right arrow Articles by Fernie, A. R.
Agricola
Right arrow Articles by Carrari, F.
Right arrow Articles by Fernie, A. R.

Received May 11, 2003
Returned for revision May 27, 2003
Accepted July 17, 2003

Reduced Expression of Aconitase Results in an Enhanced Rate of Photosynthesis and Marked Shifts in Carbon Partitioning in Illuminated Leaves of Wild Species Tomato

Fernando Carrari , Adriano Nunes-Nesi , Yves Gibon , Anna Lytovchenko , Marcelo Ehlers Loureiro , and Alisdair R. Fernie *

Department Willmitzer, Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm, Germany (F.C., A.N.-N., Y.G., A.L., A.R.F.); and Departamento de Biologia Vegetal, Federal University of Vicosa, 36571-000 Viçosa-MG, Brazil (M.E.L.)

* Corresponding author; email: fernie{at}mpimp-golm.mpg.de.

Wild species tomato (Lycopersicon pennellii) plants bearing a genetic lesion in the gene encoding aconitase (Aco-1; aconitate hydratase EC 4.2.1.3) were characterized at molecular and biochemical levels. The genetic basis of this lesion was revealed by cloning the wild-type and mutant alleles. The mutation resulted in lowered expression of the Aco-1 transcript and lowered levels of both cytosolic and mitochondrial aconitase protein and activity. After in silico analysis, we concluded that in the absence of a recognizable target sequence, the best explanation for the dual location of this protein is inefficient targeting. Biochemical analysis of leaves of the Aco-1 accession suggested that they exhibited a restricted flux through the Krebs cycle and reduced levels of Krebs cycle intermediates but were characterized by elevated adenylate levels and an enhanced rate of CO2 assimilation. Furthermore, the analysis of both steady-state metabolite levels and metabolic fluxes revealed that this accession also exhibited elevated rates of photosynthetic Suc synthesis and a corresponding increase in fruit yield. Therefore, we conclude that the Krebs cycle normally competes with the Suc synthetic pathway for carbon but is not essential for the supply of energy to fuel the operation of this pathway.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. I. Zanor, S. Osorio, A. Nunes-Nesi, F. Carrari, M. Lohse, B. Usadel, C. Kuhn, W. Bleiss, P. Giavalisco, L. Willmitzer, et al.
RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and Fertility
Plant Physiology, July 1, 2009; 150(3): 1204 - 1218.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Roschzttardtz, I. Fuentes, M. Vasquez, C. Corvalan, G. Leon, I. Gomez, A. Araya, L. Holuigue, J. Vicente-Carbajosa, and X. Jordana
A Nuclear Gene Encoding the Iron-Sulfur Subunit of Mitochondrial Complex II Is Regulated by B3 Domain Transcription Factors during Seed Development in Arabidopsis
Plant Physiology, May 1, 2009; 150(1): 84 - 95.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
I. Strodtkotter, K. Padmasree, C. Dinakar, B. Speth, P. S. Niazi, J. Wojtera, I. Voss, P. T. Do, A. Nunes-Nesi, A. R. Fernie, et al.
Induction of the AOX1D Isoform of Alternative Oxidase in A. thaliana T-DNA Insertion Lines Lacking Isoform AOX1A Is Insufficient to Optimize Photosynthesis when Treated with Antimycin A
Mol Plant, March 1, 2009; 2(2): 284 - 297.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. J. van der Merwe, S. Osorio, T. Moritz, A. Nunes-Nesi, and A. R. Fernie
Decreased Mitochondrial Activities of Malate Dehydrogenase and Fumarase in Tomato Lead to Altered Root Growth and Architecture via Diverse Mechanisms
Plant Physiology, February 1, 2009; 149(2): 653 - 669.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. L. Araujo, A. Nunes-Nesi, S. Trenkamp, V. I. Bunik, and A. R. Fernie
Inhibition of 2-Oxoglutarate Dehydrogenase in Potato Tuber Suggests the Enzyme Is Limiting for Respiration and Confirms Its Importance in Nitrogen Assimilation
Plant Physiology, December 1, 2008; 148(4): 1782 - 1796.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Palmieri, A. Santoro, F. Carrari, E. Blanco, A. Nunes-Nesi, R. Arrigoni, F. Genchi, A. R. Fernie, and F. Palmieri
Identification and Characterization of ADNT1, a Novel Mitochondrial Adenine Nucleotide Transporter from Arabidopsis
Plant Physiology, December 1, 2008; 148(4): 1797 - 1808.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. N. Oliver, J. E. Lunn, E. Urbanczyk-Wochniak, A. Lytovchenko, J. T. van Dongen, B. Faix, E. Schmalzlin, A. R. Fernie, and P. Geigenberger
Decreased Expression of Cytosolic Pyruvate Kinase in Potato Tubers Leads to a Decline in Pyruvate Resulting in an in Vivo Repression of the Alternative Oxidase
Plant Physiology, November 1, 2008; 148(3): 1640 - 1654.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. C.R. Williams, L. Miguet, S. K. Masakapalli, N. J. Kruger, L. J. Sweetlove, and R. G. Ratcliffe
Metabolic Network Fluxes in Heterotrophic Arabidopsis Cells: Stability of the Flux Distribution under Different Oxygenation Conditions
Plant Physiology, October 1, 2008; 148(2): 704 - 718.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. B. Xu, T. Li, Z. Y. Deng, K. Chong, Y. Xue, and T. Wang
Dynamic Proteomic Analysis Reveals a Switch between Central Carbon Metabolism and Alcoholic Fermentation in Rice Filling Grains
Plant Physiology, October 1, 2008; 148(2): 908 - 925.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Nunes-Nesi, R. Sulpice, Y. Gibon, and A. R. Fernie
The enigmatic contribution of mitochondrial function in photosynthesis
J. Exp. Bot., May 1, 2008; 59(7): 1675 - 1684.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Sienkiewicz-Porzucek, A. Nunes-Nesi, R. Sulpice, J. Lisec, D. C. Centeno, P. Carillo, A. Leisse, E. Urbanczyk-Wochniak, and A. R. Fernie
Mild Reductions in Mitochondrial Citrate Synthase Activity Result in a Compromised Nitrate Assimilation and Reduced Leaf Pigmentation But Have No Effect on Photosynthetic Performance or Growth
Plant Physiology, May 1, 2008; 147(1): 115 - 127.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Alhagdow, F. Mounet, L. Gilbert, A. Nunes-Nesi, V. Garcia, D. Just, J. Petit, B. Beauvoit, A. R. Fernie, C. Rothan, et al.
Silencing of the Mitochondrial Ascorbate Synthesizing Enzyme L-Galactono-1,4-Lactone Dehydrogenase Affects Plant and Fruit Development in Tomato
Plant Physiology, December 1, 2007; 145(4): 1408 - 1422.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Studart-Guimaraes, A. Fait, A. Nunes-Nesi, F. Carrari, B. Usadel, and A. R. Fernie
Reduced Expression of Succinyl-Coenzyme A Ligase Can Be Compensated for by Up-Regulation of the {gamma}-Aminobutyrate Shunt in Illuminated Tomato Leaves
Plant Physiology, November 1, 2007; 145(3): 626 - 639.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Lemaitre, E. Urbanczyk-Wochniak, V. Flesch, E. Bismuth, A. R. Fernie, and M. Hodges
NAD-Dependent Isocitrate Dehydrogenase Mutants of Arabidopsis Suggest the Enzyme Is Not Limiting for Nitrogen Assimilation
Plant Physiology, July 1, 2007; 144(3): 1546 - 1558.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Leon, L. Holuigue, and X. Jordana
Mitochondrial Complex II Is Essential for Gametophyte Development in Arabidopsis
Plant Physiology, April 1, 2007; 143(4): 1534 - 1546.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Carrari, C. Baxter, B. Usadel, E. Urbanczyk-Wochniak, M.-I. Zanor, A. Nunes-Nesi, V. Nikiforova, D. Centero, A. Ratzka, M. Pauly, et al.
Integrated Analysis of Metabolite and Transcript Levels Reveals the Metabolic Shifts That Underlie Tomato Fruit Development and Highlight Regulatory Aspects of Metabolic Network Behavior
Plant Physiology, December 1, 2006; 142(4): 1380 - 1396.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Carrari and A. R. Fernie
Metabolic regulation underlying tomato fruit development
J. Exp. Bot., June 1, 2006; 57(9): 1883 - 1897.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Li, D. W. Ehrhardt, and S. Y. Rhee
Systematic Analysis of Arabidopsis Organelles and a Protein Localization Database for Facilitating Fluorescent Tagging of Full-Length Arabidopsis Proteins
Plant Physiology, June 1, 2006; 141(2): 527 - 539.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Nunes-Nesi, F. Carrari, A. Lytovchenko, A. M.O. Smith, M. Ehlers Loureiro, R. G. Ratcliffe, L. J. Sweetlove, and A. R. Fernie
Enhanced Photosynthetic Performance and Growth as a Consequence of Decreasing Mitochondrial Malate Dehydrogenase Activity in Transgenic Tomato Plants
Plant Physiology, February 1, 2005; 137(2): 611 - 622.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Urbanczyk-Wochniak and A. R. Fernie
Metabolic profiling reveals altered nitrogen nutrient regimes have diverse effects on the metabolism of hydroponically-grown tomato (Solanum lycopersicum) plants
J. Exp. Bot., January 1, 2005; 56(410): 309 - 321.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. Schauer, D. Zamir, and A. R. Fernie
Metabolic profiling of leaves and fruit of wild species tomato: a survey of the Solanum lycopersicum complex
J. Exp. Bot., January 1, 2005; 56(410): 297 - 307.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Carrari, D. Coll-Garcia, N. Schauer, A. Lytovchenko, N. Palacios-Rojas, I. Balbo, M. Rosso, and A. R. Fernie
Deficiency of a Plastidial Adenylate Kinase in Arabidopsis Results in Elevated Photosynthetic Amino Acid Biosynthesis and Enhanced Growth
Plant Physiology, January 1, 2005; 137(1): 70 - 82.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists