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Published on November 6, 2003; 10.1104/pp.103.027367

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Received May 23, 2003
Returned for revision June 15, 2003
Accepted August 21, 2003

Expression of Arabidopsis {gamma}-Tubulin in Fission Yeast Reveals Conserved and Novel Functions of {gamma}-Tubulin

Tetsuya Horio * and Berl R. Oakley

Department of Nutrition, The University of Tokushima School of Medicine, Kuramoto, Tokushima, 770-8503, Japan (T.H.); and Department of Molecular Genetics and Department of Plant Biology, The Ohio State University, Columbus, Ohio 43210 (B.R.O)

* Corresponding author; email: horio{at}nutr.med.tokushima-u.ac.jp.

{gamma}-Tubulin localizes to microtubule-organizing centers in animal and fungal cells where it is important for microtubule nucleation. Plant cells do not have morphologically defined microtubule organizing centers, however, and {gamma}-tubulin is distributed in small, discrete structures along microtubules. The great difference in distribution has prompted speculation that plant {gamma}-tubulins function differently from animal and fungal {gamma}-tubulins. We tested this possibility by expressing Arabidopsis {gamma}-tubulin in the fission yeast Schizosaccharomyces pombe. At high temperatures, the plant {gamma}-tubulin was able to bind to microtubule-organizing centers, nucleate microtubule assembly, and support the growth and replication of S. pombe cells lacking endogenous {gamma}-tubulin. However, the distribution of microtubules was abnormal as was cell morphology, and at low temperatures, cells were arrested in mitosis. These results reveal that Arabidopsis {gamma}-tubulin can carry out essential functions in S. pombe and is, thus, functionally conserved. The morphological abnormalities reveal that it cannot carry out some nonessential functions, however, and they underscore the importance of {gamma}-tubulin in morphogenesis of fission yeast cells and in maintaining normal interphase microtubule arrays.




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