Plant Physiology Preview
Published on December 4, 2003; 10.1104/pp.103.027466
Received May 27, 2003
Returned for revision August 12, 2003
Accepted October 10, 2003
An Invertase Inhibitor from Maize Localizes to the Embryo Surrounding Region during EarlyKernel Development
Nicholas J. Bate *, Xiping Niu , Yuwen Wang , Kellie S. Reimann , and Timothy G. Helentjaris
Agronomic Traits, Trait and Technology Development, Pioneer Hi-Bred International, 7250 N.W. 62nd Avenue, Johnston, Iowa, 50131-0552
* Corresponding author; email: nic.bate{at}pioneer.com.
Invertase activity is thought to play a regulatory role during early kernel development by converting sucrose originating from source leaves into hexoses to support cell division in the endosperm and embryo. Invertases are regulated at the posttranslational level by small protein inhibitors, INVINHs. We found that in maize (Zea mays), an invertase inhibitor homolog (ZM-INVINH1) is expressed early in kernel development, between 4 and 7 d after pollination. Invertase activity is reduced in vitro in the presence of recombinant ZM-INVINH1, and inhibition is attenuated by pre-incubation with sucrose. The presence of a putative signal peptide, fractionation experiments, and ZM-INVINH1::green fluorescent protein fusion experiments indicate that the protein is exported to the apoplast. Moreover, association of ZM-INVINH1 with the glycoprotein fraction by concanavalin A chromatogaphy suggests that ZM-INVINH1 interacts with an apoplastic invertase during early kernel development. ZM-INVINH1 was localized to the embryo surrounding region by in situ analysis, suggesting that this region forms a boundary, compartmentalizing apoplast invertase activity to allow different embryo and endosperm developmental rates.
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