Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on December 11, 2003; 10.1104/pp.103.027615

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
134/1/237    most recent
pp.103.027615v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sergeeva, L. I.
Right arrow Articles by Vreugdenhil, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sergeeva, L. I.
Right arrow Articles by Vreugdenhil, D.
Agricola
Right arrow Articles by Sergeeva, L. I.
Right arrow Articles by Vreugdenhil, D.

Received May 27, 2003
Returned for revision July 30, 2003
Accepted October 9, 2003

Histochemical Analysis Reveals Organ-Specific Quantitative Trait Loci for Enzyme Activities in Arabidopsis

Lidiya I. Sergeeva , Jenneke Vonk , Joost J.B. Keurentjes , Linus H.W. van der Plas , Maarten Koornneef , and Dick Vreugdenhil *

Laboratory of Plant Physiology (L.I.S., J.V., J.J.B.K., L.H.W.v.d.P., D.V.) and Laboratory of Genetics (J.J.B.K., M.K.), Graduate School Experimental Plant Sciences, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands; and Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127 276, Moscow, Russia (L.I.S.)

* Corresponding author; email: dick.vreugdenhil{at}wur.nl.

To identify genetic loci involved in the regulation of organ-specific enzyme activities, a specific histochemical staining protocol was used in combination with quantitative trait locus (QTL) analysis. Using phosphoglucomutase (PGM) as an example, it is shown that enzyme activity can specifically, and with high resolution, be visualized in non-sectioned seedlings of Arabidopsis. The intensities of staining were converted to quantitative data and used as trait for QTL analysis using Landsberg erecta x Cape Verde Islands recombinant inbred lines. Independently, PGM activities were quantified in whole-seedling extracts, and these data were also used for QTL analysis. On the basis of extract data, six significant (P < 0.05) loci affecting PGM activity were found. From the histochemical data, one or more specific QTLs were found for each organ analyzed (cotyledons, shoot apex, hypocotyl, root, root neck, root tip, and root hairs). Loci detected for PGM activity in extracts colocated with loci for histochemical staining. QTLs were found coinciding with positions of (putative) PGM genes but also at other positions, the latter ones supposedly pointing toward regulatory genes. Some of this type of loci were also organ specific. It is concluded that QTL analysis based on histochemical data is feasible and may reveal organ-specific loci involved in the regulation of metabolic pathways.




This article has been cited by other articles:


Home page
 Plant CellHome page
C. Alonso-Blanco, M. G.M. Aarts, L. Bentsink, J. J.B. Keurentjes, M. Reymond, D. Vreugdenhil, and M. Koornneef
What Has Natural Variation Taught Us about Plant Development, Physiology, and Adaptation?
PLANT CELL, July 1, 2009; 21(7): 1877 - 1896.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
DanielJ. Kliebenstein
Advancing Genetic Theory and Application by Metabolic Quantitative Trait Loci Analysis
PLANT CELL, June 1, 2009; 21(6): 1637 - 1646.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. M. Cross, M. von Korff, T. Altmann, L. Bartzetko, R. Sulpice, Y. Gibon, N. Palacios, and M. Stitt
Variation of Enzyme Activities and Metabolite Levels in 24 Arabidopsis Accessions Growing in Carbon-Limited Conditions
Plant Physiology, December 1, 2006; 142(4): 1574 - 1588.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. I. Sergeeva, J. J. B. Keurentjes, L. Bentsink, J. Vonk, L. H. W. van der Plas, M. Koornneef, and D. Vreugdenhil
Vacuolar invertase regulates elongation of Arabidopsis thaliana roots as revealed by QTL and mutant analysis
PNAS, February 21, 2006; 103(8): 2994 - 2999.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. C. G. Wheeler, M. A. Tronconi, M. F. Drincovich, C. S. Andreo, U.-I. Flugge, and V. G. Maurino
A Comprehensive Analysis of the NADP-Malic Enzyme Gene Family of Arabidopsis
Plant Physiology, September 1, 2005; 139(1): 39 - 51.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists