Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 2, 2003; 10.1104/pp.103.027797

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
133/2/630    most recent
pp.103.027797v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sakr, S.
Right arrow Articles by Chrispeels, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakr, S.
Right arrow Articles by Chrispeels, M. J.
Agricola
Right arrow Articles by Sakr, S.
Right arrow Articles by Chrispeels, M. J.

Received May 30, 2003
Returned for revision June 30, 2003
Accepted June 30, 2003

Plasma Membrane Aquaporins Are Involved in Winter Embolism Recovery in Walnut Tree

Soulaiman Sakr *, Georges Alves , Raphaël Morillon , Karine Maurel , Mélanie Decourteix , Agnès Guilliot , Pierrette Fleurat-Lessard , Jean-Louis Julien , and Maarten J. Chrispeels

Unité Mixte de Recherche 547-Physiologie Integreé de d'Arbre Fruitier Institut National de la Recherche Agronomique, Site des Cézeaux, Université Blaise Pascal, 24 Avenue des Landais, 63177Aubière cedex, France (S.S., G.A., K.M., M.D., A.G., J.-L.J.); Division of Biological Sciences, University of California San Diego, La Jolla, California 92093-0116 (R.M., M.J.C.); and Unité Mixte de Recherche Centre National de la Recherche Scientifique 6161, Bâtiment Botanique, 40 Avenue Recteur Pineau, 86022 Poitiers, France (P.F.-L.)

* Corresponding author; email: Soulaiman.Sakr{at}piaf.univbpclermont.fr.

In perennial plants, freeze-thaw cycles during the winter months can induce the formation of air bubbles in xylem vessels, leading to changes in their hydraulic conductivity. Refilling of embolized xylem vessels requires an osmotic force that is created by the accumulation of soluble sugars in the vessels. Low water potential leads to water movement from the parenchyma cells into the xylem vessels. The water flux gives rise to a positive pressure essential for the recovery of xylem hydraulic conductivity. We investigated the possible role of plasma membrane aquaporins in winter embolism recovery in walnut (Juglans regia). First, we established that xylem parenchyma starch is converted to sucrose in the winter months. Then, from a xylem-derived cDNA library, we isolated two PIP2 aquaporin genes (JrPIP2,1 and JrPIP2,2) that encode nearly identical proteins. The water channel activity of the JrPIP2,1 protein was demonstrated by its expression in Xenopus laevis oocytes. The expression of the two PIP2 isoforms was investigated throughout the autumn-winter period. In the winter period, high levels of PIP2 mRNA and corresponding protein occurred simultaneously with the rise in sucrose. Furthermore, immunolocalization studies in the winter period show that PIP2 aquaporins were mainly localized in vessel-associated cells, which play a major role in controlling solute flux between parenchyma cells and xylem vessels. Taken together, our data suggest that PIP2 aquaporins could play a role in water transport between xylem parenchyma cells and embolized vessels.




This article has been cited by other articles:


Home page
 J Exp BotHome page
M. C. Voicu, J. E. K. Cooke, and J. J. Zwiazek
Aquaporin gene expression and apoplastic water flow in bur oak (Quercus macrocarpa) leaves in relation to the light response of leaf hydraulic conductance
J. Exp. Bot., October 1, 2009; 60(14): 4063 - 4075.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Tungngoen, P. Kongsawadworakul, U. Viboonjun, M. Katsuhara, N. Brunel, S. Sakr, J. Narangajavana, and H. Chrestin
Involvement of HbPIP2;1 and HbTIP1;1 Aquaporins in Ethylene Stimulation of Latex Yield through Regulation of Water Exchanges between Inner Liber and Latex Cells in Hevea brasiliensis
Plant Physiology, October 1, 2009; 151(2): 843 - 856.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. Dusotoit-Coucaud, N. Brunel, P. Kongsawadworakul, U. Viboonjun, A. Lacointe, J.-L. Julien, H. Chrestin, and S. Sakr
Sucrose importation into laticifers of Hevea brasiliensis, in relation to ethylene stimulation of latex production
Ann. Bot., September 1, 2009; 104(4): 635 - 647.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G.-W. Li, M.-H. Zhang, W.-M. Cai, W.-N. Sun, and W.-A. Su
Characterization of OsPIP2;7, a Water Channel Protein in Rice
Plant Cell Physiol., December 1, 2008; 49(12): 1851 - 1858.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Cochard, J.-S. Venisse, T. S. Barigah, N. Brunel, S. Herbette, A. Guilliot, M. T. Tyree, and S. Sakr
Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light
Plant Physiology, January 1, 2007; 143(1): 122 - 133.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. M. Herman, K. Rotter, R. Premakumar, G Elwinger, R. Bae, L. Ehler-King, S. Chen, and D. P. Livingston III
Additional freeze hardiness in wheat acquired by exposure to -3 {degrees}C is associated with extensive physiological, morphological, and molecular changes
J. Exp. Bot., November 1, 2006; 57(14): 3601 - 3618.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists